Document Detail

Evaluation of a real-time polymerase chain reaction (PCR) assay for detection of anisakis simplex parasite as a food-borne allergen source in seafood products.
MedLine Citation:
PMID:  20085278     Owner:  NLM     Status:  MEDLINE    
Anisakis simplex has been recognized as an important cause of disease in humans and as a food-borne allergen source. Actually, this food-borne parasite was recently identified as an emerging food safety risk. An A. simplex -specific primer-probe system based on a real-time polymerase chain reaction (PCR) detection assay has been successfully optimized and validated with seafood samples. In addition, a DNA extraction procedure has been optimized to detect the presence of the nematode in food samples. The assay is a very reliable, specific, and sensitive methodology to detect the presence of traces of this parasite in seafood products, including highly processed samples. As a result, 13 sequences of cytochrome c oxidase II gene were obtained and scrutinized to calculate intra- and interspecific variabilities of 0 and 35-67%, respectively. Finally, an efficiency of 2.07 +/- 0.14 of the assay was calculated, and a limit of detection of 40 ppm parasite in 25 g of sample was also optimized. Actually, the presence of this parasite in several seafood products has been demonstrated, enforcing the necessity of a design for a good manufacturing practice protocol for the processing industry to minimize the presence of this parasite as a food-borne allergen source in seafood products.
Itziar Lopez; Miguel Angel Pardo
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  58     ISSN:  1520-5118     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-02-03     Completed Date:  2010-04-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1469-77     Citation Subset:  IM    
Food Research Division, Fisheries and Food Technological Institute, Parque Tecnologico de Bizkaia, Astondo Bidea-Edificio 609, E-48160 Derio, Bizkaia, Spain.
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MeSH Terms
Amino Acid Sequence
Anisakis / chemistry,  genetics,  immunology,  isolation & purification*
Antigens, Helminth / chemistry,  genetics*,  immunology
DNA Primers / genetics
Electron Transport Complex IV / chemistry,  genetics,  immunology
Fishes / parasitology*
Limit of Detection
Molecular Sequence Data
Polymerase Chain Reaction / methods*
Seafood / analysis*,  parasitology
Sequence Alignment
Reg. No./Substance:
0/Antigens, Helminth; 0/DNA Primers; EC 1.9.3.-/cytochrome C oxidase subunit II; EC Transport Complex IV

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