Document Detail


Evaluation of a real-time PCR assay for the detection and quantification of Bacillus cereus group spores in food.
MedLine Citation:
PMID:  20819358     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A procedure based on quantitative real-time PCR was evaluated for the detection and quantification of Bacillus cereus spores. Several methods for DNA isolation, such as various heat treatments and germination solutions, were evaluated on spore suspensions of representative strains of the B. cereus group. Overall, the commercially available DNeasy tissue kit yielded the maximum amount of DNA. The procedure also was used to construct calibration curves for different food matrices, with a wide spore quantification range of 5 log units using serial dilutions of spore suspensions of B. cereus CECT 148T. The detection limit for B. cereus in artificially contaminated liquid egg and reconstituted infant formula was about 4 spores per reaction or 60 spores per ml. The newly developed methodology based on the DNeasy tissue kit and an SYBR Green quantitative real-time PCR assay is very suitable for the rapid and accurate detection and quantification of B. cereus group strains and their spores in food samples.
Authors:
Juan Francisco Martínez-Blanch; Gloria Sánchez; Esperanza Garay; Rosa Aznar
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of food protection     Volume:  73     ISSN:  0362-028X     ISO Abbreviation:  J. Food Prot.     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-09-07     Completed Date:  2010-10-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7703944     Medline TA:  J Food Prot     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1480-5     Citation Subset:  IM    
Affiliation:
Spanish Type Culture Collection, Department of Microbiology and Ecology, University of Valencia, Valencia, Spain.
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MeSH Terms
Descriptor/Qualifier:
Bacillus cereus / isolation & purification*,  physiology*
Calibration
Colony Count, Microbial
DNA, Bacterial / analysis*
Food Contamination / analysis*
Food Microbiology
Humans
Infant
Infant Formula
Infant, Newborn
Kinetics
Polymerase Chain Reaction*
Spores, Bacterial / growth & development,  isolation & purification
Chemical
Reg. No./Substance:
0/DNA, Bacterial

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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