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Evaluation of Phenotypic and Genotypic Approaches for the Detection of Class A and Class B Carbapenemases in Enterobacteriaceae.
MedLine Citation:
PMID:  23336577     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The spread of carbapenemases in Enterobacteriaceae is among the most important issues in the antimicrobial resistance. The rapid and recent diffusion of class A and B carbapenemases determined the need of specific diagnostic tests able to detect with high sensitivity this type of resistance and to discriminate between the different enzymes. The aim of this study was to test two carbapenemase detection assays, the Rosco Synergic and the Hyplex polymerase chain reaction-enzyme-linked immunosorbent assays for screening carbapenemase-producing Enterobacteriaceae. The phenotypic and genotypic tests were evaluated among 108 clinical isolates, including Klebsiella pneumoniae carbapenemase (KPC) (n=50) and metallo-β-lactamase- (MBL) (n=20), and AmpC- (n=10) producing Enterobacteriaceae. The commercial phenotypic assay showed a high sensitivity performance detecting all KPC and MBL producers, including New Delhi MBL 1 (NDM-1) strains. In addition, the Rosco Synergic assay was able to distinguish specifically between the different mechanisms that confer resistance to carbapenems in Enterobacteriaceae. We also demonstrated that the genotypic test was able to detect all the class A and B carbapenemases showing high sensitivity (100%) and specificity (98%) in a fast and reliable time. Based on these results, both the commercial phenotypic and the genotypic assays could be helpful as confirmatory and discriminatory tests for the detection of class A and class B carbapenemases.
Authors:
Simone Ambretti; Paolo Gaibani; Andrea Berlingeri; Miriam Cordovana; Maria Vittoria Tamburini; Gloria Bua; Maria Paola Landini; Vittorio Sambri
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-22
Journal Detail:
Title:  Microbial drug resistance (Larchmont, N.Y.)     Volume:  -     ISSN:  1931-8448     ISO Abbreviation:  Microb. Drug Resist.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-22     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9508567     Medline TA:  Microb Drug Resist     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Operative Unit of Clinical Microbiology, S. Orsola-Malpighi University Hospital , Bologna, Italy .
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