Document Detail


Evaluation of the petrifilm aerobic count plate for enumeration of aerobic marine bacteria from seawater and Caulerpa lentillifera.
MedLine Citation:
PMID:  20819367     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The enumeration and evaluation of the activity of marine bacteria are important in the food industry. However, detection of marine bacteria in seawater or seafood has not been easy. The Petrifilm aerobic count plate (ACP) is a ready-to-use alternative to the traditional enumeration media used for bacteria associated with food. The purpose of this study was to evaluate the usefulness of a simple detection and enumeration method utilizing the Petrifilm ACP for enumeration of aerobic marine bacteria from seawater and an edible seaweed, Caulerpa lentillifera. The efficiency of enumeration of total aerobic marine bacteria on Petrifilm ACP was compared with that using the spread plate method on marine agar with 80 seawater and 64 C. lentillifera samples. With sterile seawater as the diluent, a close correlation was observed between the method utilizing Petrifilm ACP and that utilizing the conventional marine agar (r=0.98 for seawater and 0.91 for C. lentillifera). The Petrifilm ACP method was simpler and less time-consuming than the conventional method. These results indicate that Petrifilm ACP is a suitable alternative to conventional marine agar for enumeration of marine microorganisms in seawater and C. lentillifera samples.
Authors:
Jun Kudaka; Toru Horii; Koji Tamanaha; Kiyomasa Itokazu; Masaji Nakamura; Katsuya Taira; Minoru Nidaira; Sho Okano; Akio Kitahara
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of food protection     Volume:  73     ISSN:  0362-028X     ISO Abbreviation:  J. Food Prot.     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-09-07     Completed Date:  2010-10-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7703944     Medline TA:  J Food Prot     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1529-32     Citation Subset:  IM    
Affiliation:
Okinawa Prefectural Institute Health and Environment, 2085 Ozato, Nanjo-shi Okinawa 901-1202, Japan. kudakajn@pref.okinawa.lg.jp
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MeSH Terms
Descriptor/Qualifier:
Bacteria, Aerobic / isolation & purification*
Bacteriological Techniques / instrumentation,  methods
Caulerpa / microbiology*
Colony Count, Microbial / instrumentation,  methods,  standards*
Consumer Product Safety
Food Microbiology
Humans
Seawater / microbiology*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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