| Evaluation of a Multi-layer Adipose-Derived Stem Cell Sheet in a Full-thickness Wound Healing Model. | |
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MedLine Citation:
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PMID: 23022891 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Cell sheet technology has been studied for applications such as bone, ligament, and skin regeneration. There has been limited examination of adipose-derived stem cells (ASCs) for cell sheet applications. The specific aim of this study was to evaluate ASC sheet technology for wound healing. ASCs were isolated from discarded human abdominal subcutaneous adipose tissue, and ASC cell sheets were created on the surface of fibrin-grafted culture dishes. In vitro examination consisted of the histochemical characterization of the ASC sheets. In vivo experiments consisted of implanting single layer cell sheets, triple layer cell sheets, or non-treated control onto a full-thickness wound defect (include epidermis, dermis, and subcutaneous fat) in nude mice for three weeks. Cell sheets were easily peeled off of the culture dishes using forceps. The single layer and triple layer ASC sheets showed complete extracellular structure via hematoxylin and eosin (H and E) staining. In vivo, the injury area was measured 7, 10, 14, and 21 days post treatment to assess wound recovery. The ASC sheet-treated groups' injury area was significantly smaller than that of the non-treated control group at all time points, except day 21 The triple layer ASC sheet treatment significantly enhanced wound healing compared to the single layer ASC sheet at 7, 10, and 14 days. The density of blood vessel showed that ASC cell sheet treatment slightly enhanced total vessel proliferation compared to the empty wound injury treatment. Our studies indicate that ASC sheets present a potentially viable matrix for full-thickness defect wound healing in a mouse model. Consequently, our ASC sheet technology represents a substantial advance in developing various types of three-dimensional tissues. |
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Authors:
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Yen-Chih Lin; Tara Grahovac; Sun Jung Oh; Matthew Ieraci; J Peter Rubin; Kacey G Marra |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-9-27 |
Journal Detail:
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Title: Acta biomaterialia Volume: - ISSN: 1878-7568 ISO Abbreviation: Acta Biomater Publication Date: 2012 Sep |
Date Detail:
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Created Date: 2012-10-1 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101233144 Medline TA: Acta Biomater Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2012. Published by Elsevier Ltd. |
Affiliation:
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Department of Plastic and Reconstructive Surgery, University of Pittsburgh, Pittsburgh, PA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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