Document Detail


Evaluation of mucoadhesive PLGA microparticles for nasal immunization.
MedLine Citation:
PMID:  20077052     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this study, hepatitis B surface antigen (HBsAg) loaded poly(lactic-co-glycolic acid) (PLGA) microparticles were prepared and coated with chitosan and trimethyl chitosan (TMC) to evaluate the effect of coating material for nasal vaccine delivery. The developed formulations were characterized for size, zeta potential, entrapment efficiency, and mucin adsorption ability. Plain PLGA microparticles demonstrated negative zeta potential. However, coated microparticles showed higher positive zeta potential. Results indicated that TMC microparticles demonstrated substantially higher mucin adsorption when compared to chitosan-coated microparticles and plain PLGA microparticles. The coated and uncoated microparticles showed deposition in nasal-associated lymphoid tissue under fluorescence microscopy. The coated and uncoated microparticles were then administered intranasally to mice. Immune-adjuvant effect was determined on the basis of specific antibody titer observed in serum and secretions using enzyme-linked immunosorbent assay. It was observed that coated particles showed a markedly increased anti-HBsAg titer as compared to plain PLGA microparticles, but the results were more pronounced with the TMC-coated PLGA microparticles.
Authors:
Dilip Pawar; Amit K Goyal; Sharad Mangal; Neeraj Mishra; Bhuvaneshwar Vaidya; Shailja Tiwari; Arvind K Jain; Suresh P Vyas
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-01-15
Journal Detail:
Title:  The AAPS journal     Volume:  12     ISSN:  1550-7416     ISO Abbreviation:  AAPS J     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-03-24     Completed Date:  2010-06-15     Revised Date:  2013-05-31    
Medline Journal Info:
Nlm Unique ID:  101223209     Medline TA:  AAPS J     Country:  United States    
Other Details:
Languages:  eng     Pagination:  130-7     Citation Subset:  IM    
Affiliation:
Drug Delivery Research Laboratory, Department of Pharmaceutical Sciences, Dr. H. S. Gour University, Sagar, MP 470 003, India.
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MeSH Terms
Descriptor/Qualifier:
Administration, Intranasal
Animals
Chemistry, Pharmaceutical
Chitosan
Drug Carriers
Electrochemistry
Enzyme-Linked Immunosorbent Assay
Excipients / chemistry*
Female
Hepatitis B Surface Antigens / administration & dosage,  immunology
Immunoglobulin A / analysis,  biosynthesis
Immunoglobulin G / analysis,  biosynthesis
Lactic Acid / chemistry*
Mice
Mice, Inbred BALB C
Microscopy, Electron, Scanning
Microscopy, Fluorescence
Mucins / chemistry
Nanoparticles
Polyglycolic Acid / chemistry*
Tissue Adhesives
Vaccines / administration & dosage*
Chemical
Reg. No./Substance:
0/Drug Carriers; 0/Excipients; 0/Hepatitis B Surface Antigens; 0/Immunoglobulin A; 0/Immunoglobulin G; 0/Mucins; 0/N-trimethyl chitosan chloride; 0/Tissue Adhesives; 0/Vaccines; 0/polylactic acid-polyglycolic acid copolymer; 26009-03-0/Polyglycolic Acid; 50-21-5/Lactic Acid; 9012-76-4/Chitosan
Comments/Corrections

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