Document Detail


Evaluation of Matrex cellufine GH 25.
MedLine Citation:
PMID:  12834969     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The aim of this work was to test a new matrix for group size exclusion chromatography, Matrex cellufine GH 25, and compare it with Sephadex G25 Superfine and Sephadex G25 fine. Matrex cellufine GH 25 showed a better behaviour at high flow rate (792 cm/h) without back-pressure or packing-down. Sephadex G25 superfine showed a limited flow rate (226 cm/h) with both back-pressure and packing-down and Sephades G25 fine allowed running at 792 cm/h but with packing-down fourfold superior to that of Matrex cellufine GH 25. To have the same number of theoretical plates, it was necessary to increase the height of the bed and therefore the volume of the matrix (43%). With Matrex cellufine GH 25, the sample volume was more limited (9% of column volume) than with Sephadex G25 superfine (17%) but was equivalent to Sephadex fine (9%). To have the same elution time as Sephadex G25 superfine, the flow rate had to be increased by a factor 1.7. As Matrex cellufine GH 25 allowed a high flow rate when the volume of the sample was limited to 6% of column volume, its performance is better than that of Sephadex G25.
Authors:
Patrick Vincent; Jean-Pierre Compoint; Valérie Fitton; Xavier Santarelli
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Publication Detail:
Type:  Comparative Study; Evaluation Studies; Journal Article; Validation Studies    
Journal Detail:
Title:  Journal of biochemical and biophysical methods     Volume:  56     ISSN:  0165-022X     ISO Abbreviation:  J. Biochem. Biophys. Methods     Publication Date:  2003 Jun 
Date Detail:
Created Date:  2003-07-01     Completed Date:  2004-05-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7907378     Medline TA:  J Biochem Biophys Methods     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  69-78     Citation Subset:  IM    
Affiliation:
Ecole Supérieure de Technologie des Biomolécules de Bordeaux (ESTBB), UMR 5544, Université Victor Segalen Bordeaux 2, 146 rue Léo Saignat, 33076 Bordeaux Cedex, France.
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MeSH Terms
Descriptor/Qualifier:
Chromatography, Gel / instrumentation*,  methods*
Dextrans / chemistry*
Gels / chemistry*
Materials Testing*
Microfluidics / instrumentation,  methods*
Pressure
Sensitivity and Specificity
Serum Albumin, Bovine / isolation & purification*
Ultrafiltration / instrumentation*,  methods*
Chemical
Reg. No./Substance:
0/Gels; 0/Serum Albumin, Bovine; 9004-54-0/Dextrans; 9014-76-0/sephadex

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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