Document Detail


Evaluation of MYC and chromosome 8 copy number in breast carcinoma by interphase cytogenetics.
MedLine Citation:
PMID:  8993975     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We used fluorescence in situ hybridization (FISH) to determine MYC and chromosome 8 copy number on whole nuclear imprint preparations of 24 breast carcinomas, seven benign breast samples, and two phyllodes tumors. None of the benign tissues and neither of the phyllodes tumors demonstrated an increased copy number for MYC or chromosome 8, which was defined as greater than two signals in > 10% of nuclei. In contrast, 22 of 24 carcinomas demonstrated an increased MYC copy number. The modal numbers of MYC copies/nucleus were 0-2 in seven cases (29%), 3-5 in seven cases (29%), 6-9 in five cases (21%), and > 9 in five cases (21%). An increased chromosome 8 copy number was observed in 21 of 22 carcinomas with MYC gain, and the modal number of signals/nucleus was either identical to (n = 14; 64%) or less than (n = 8; 36%) the number of MYC copies. The number of MYC copies correlated with cellular DNA content, as determined by using flow cytometry. In peridiploid tumors (DNA index 0.9-1.2; n = 7), the MYC copy numbers/nucleus were 0-2 in five cases and 3-5 in two cases. In contrast, the modal MYC copy numbers/nucleus among the 11 hyperdiploid tumors (DNA index 1.3-1.9) were 0-2 in one case, 3-5 in four cases, 6-9 in five cases, and > 9 in one case. All three tetraploid/hypertetraploid carcinomas exhibited > 9 MYC copies/nucleus. We conclude that an increased MYC copy number, as detected by using interphase cytogenetics, is extremely frequent in human breast carcinomas. However, in most cases, MYC gene duplication is probably secondary to polysomy of chromosome 8 and/or genomic endoreduplication (i.e., DNA aneuploidy).
Authors:
D W Visscher; T Wallis; S Awussah; A Mohamed; J D Crissman
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Genes, chromosomes & cancer     Volume:  18     ISSN:  1045-2257     ISO Abbreviation:  Genes Chromosomes Cancer     Publication Date:  1997 Jan 
Date Detail:
Created Date:  1997-03-10     Completed Date:  1997-03-10     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  9007329     Medline TA:  Genes Chromosomes Cancer     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1-7     Citation Subset:  IM    
Affiliation:
Department of Pathology, Harper Hospital, Detroit, Michigan 48201, USA.
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MeSH Terms
Descriptor/Qualifier:
Aneuploidy
Breast Neoplasms / genetics*,  pathology
Chromosomes, Human, Pair 8* / genetics
DNA, Neoplasm / analysis*
Flow Cytometry
Gene Amplification
Genes, myc* / genetics
Humans
In Situ Hybridization, Fluorescence
Interphase
Phyllodes Tumor / genetics,  pathology
Ploidies
Chemical
Reg. No./Substance:
0/DNA, Neoplasm

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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