Document Detail


Evaluation of a Lyme disease enzyme immunoassay using the 41-G fragment of flagellin.
MedLine Citation:
PMID:  7588849     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An immunogenic region of the Borrelia burgdorferi flagellin encompassing amino acids 197-273 and designated 41-G was evaluated as an antigen in an enzyme immunoassay (EIA) for Lyme disease on a routine basis in a reference laboratory. Sera that tested positive for Lyme disease by EIA using 41-G or the whole-cell Borrelia burgdorferi lysate as the antigen were also evaluated by immunoblot for reactivity with Borrelia burgdorferi, and the patient's clinical history was determined retrospectively by a questionnaire distributed to the referring physician. The sensitivity of the 41-G based EIA for the serologic diagnosis of Lyme disease, when compared with that of the Borrelia burgdorferi lysate EIA, was 70% (35 of 50). These data demonstrate that 41-G has utility as an antigen in EIA, although the sensitivity is at present less than that of the assay employing the Borrelia burgdorferi whole-cell lysate.
Authors:
S Cretella; S Gordon; R A Flavell; E Fikrig
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology     Volume:  14     ISSN:  0934-9723     ISO Abbreviation:  Eur. J. Clin. Microbiol. Infect. Dis.     Publication Date:  1995 Jul 
Date Detail:
Created Date:  1995-12-13     Completed Date:  1995-12-13     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8804297     Medline TA:  Eur J Clin Microbiol Infect Dis     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  609-13     Citation Subset:  IM    
Affiliation:
Section of Rheumatology, Yale University School of Medicine, New Haven, Connecticut 06520-8031, USA.
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MeSH Terms
Descriptor/Qualifier:
Antigens, Bacterial / immunology*
Borrelia burgdorferi Group / immunology*
Flagellin / immunology*
Humans
Immunoenzyme Techniques
Lyme Disease / diagnosis*
Peptide Fragments / immunology*
Serologic Tests
Grant Support
ID/Acronym/Agency:
AI-30548/AI/NIAID NIH HHS; AR40452/AR/NIAMS NIH HHS; U5-CCU-106581//PHS HHS
Chemical
Reg. No./Substance:
0/Antigens, Bacterial; 0/Peptide Fragments; 12777-81-0/Flagellin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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