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Evaluation of Lipid Oxidative Stress Status in Sjogren Syndrome patients.
MedLine Citation:
PMID:  23150615     Owner:  NLM     Status:  Publisher    
PURPOSE: To evaluate the levels of lipid oxidative stress markers and inflammatory cells from tears and conjunctiva of patients with Sjogren Syndrome (SS) and normal subjects. METHODS: Thirty-one eyes of 16 patients (16 females) with SS and 15 eyes of 10 healthy controls (2 males and 8 females) were examined in this prospective study. All subjects underwent Schirmer test, tear break up time, vital stainings, confocal microscopy of the conjunctiva, tear collection for HEL (hexanoyl-lysine) ELISA and conjunctival brush cytology. Brush cytology samples underwent immunohistochemistry (IHC) staining with HEL and 4HNE (4-hydroxy-2-nonenal). Haematoxylin-Eosin and IHC staining with HEL, 4HNE were also performed on conjunctival samples of SS patients and controls. RESULTS: The tear stability and vital staining scores were significantly worse in eyes of SS patients compared to controls. Conjunctival inflammatory cell density was significantly higher in SS subjects compared to controls. The numbers of conjunctival cells positively stained for HEL and 4HNE were significantly higher in SS patients compared with controls. Tear HEL concentrations correlated significantly with staining scores and inflammatory cell density in confocal microscopy. Conjunctival specimens revealed higher numbers of cells positively stained for inflammatory markers as well as HEL and 4HNE in the IHC stainings. CONCLUSION: Increase of the oxidative stress status in the conjunctiva of SS patients appears to have a role in the pathogenesis of the dry eye disease. A close relationship may exist between ROS production, lipid peroxidation related membrane damage and inflammatory processes in dry eye.
Tais H Wakamatsu; Murat Dogru; Yukihiro Matsumoto; Takashi Kojima; Minako Kaido; Osama M A Ibrahim; Enrique A Sato; Ayako Igarashi; Yoshiyuki Ichihashi; Yoshiyuki Satake; Jun Shimazaki; Kazuo Tsubota
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-13
Journal Detail:
Title:  Investigative ophthalmology & visual science     Volume:  -     ISSN:  1552-5783     ISO Abbreviation:  Invest. Ophthalmol. Vis. Sci.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7703701     Medline TA:  Invest Ophthalmol Vis Sci     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Department of Ophthalmology, Keio University School of Medicine, Tokyo, Japan.
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