Document Detail

Evaluation of the Kudoh swab method for the culturing of Mycobacterium tuberculosis in rural areas.
MedLine Citation:
PMID:  19302632     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To compare the simple, swab 'Kudoh method' for culturing Myobacterium tuberculosis from sputum samples, to the standard Petroff digestion-decontamination procedure. The Kudoh method, which requires no centrifugation and takes only 4-5 min per sample, was also evaluated for its performance in a rural setting. METHODS: Two hospital laboratories in Caracas, Venezuela processed 314 sputum samples, in parallel, with both methods. Separately, sputum specimens were cultured with the Kudoh swab method in a field environment with minimal laboratory facilities. RESULTS: In the hospital laboratories, the sensitivity of the Kudoh swab method was comparable to that of the standard Petroff culture procedure. The swab method also performed satisfactorily in the field, improving the diagnostic sensitivity by 21% over microscopic examination alone. CONCLUSION: The Kudoh swab method is an acceptable alternative for culturing mycobacteria that is particularly suitable for rural laboratories lacking adequate infrastructure for the Petroff method.
Rossana C Jaspe; Yeimy M Rojas; Lalis A Flores; E Sofia Toro; Howard Takiff; Jacobus H de Waard
Publication Detail:
Type:  Journal Article; Multicenter Study; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Tropical medicine & international health : TM & IH     Volume:  14     ISSN:  1365-3156     ISO Abbreviation:  Trop. Med. Int. Health     Publication Date:  2009 Apr 
Date Detail:
Created Date:  2009-03-23     Completed Date:  2009-06-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9610576     Medline TA:  Trop Med Int Health     Country:  England    
Other Details:
Languages:  eng     Pagination:  468-71     Citation Subset:  IM    
Laboratorio de Tuberculosis, Universidad Central de Venezuela, Hospital Vargas, San José, Venezuela.
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MeSH Terms
Bacteriological Techniques / methods*
Culture Media*
Mycobacterium tuberculosis / growth & development,  isolation & purification*
Sensitivity and Specificity
Sputum / microbiology*
Tuberculosis / diagnosis
Reg. No./Substance:
0/Culture Media

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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