Document Detail

Evaluation of inflammatory response to acute ischemia using near-infrared fluorescent reactive oxygen sensors.
MedLine Citation:
PMID:  23378226     Owner:  NLM     Status:  MEDLINE    
PURPOSE: Ischemia-related processes associated with the generation of inflammatory molecules such as reactive oxygen species (ROS) are difficult to detect at the acute stage before the physiologic and anatomic evidence of tissue damage is present. Evaluation of the inflammatory and healing response early after an ischemic event in vivo will aid in treatment selection and patient outcomes. We introduce a novel near-infrared hydrocyanine molecular probe for the detection of ROS as a marker of tissue response to ischemia and a precursor to angiogenesis and remodeling. The synthesized molecular probe, initially a non-fluorescent hydrocyanine conjugated to polyethylene glycol, converts to a highly fluorescent cyanine reporter upon oxidation.
PROCEDURES: The probe was applied in a preclinical mouse model for myocardial infarction, where ligation and removal of a portion of the femoral artery in the hindlimb resulted in temporary ischemia followed by angiogenesis and healing.
RESULTS: The observed increase in fluorescence intensity was approximately sixfold over 24 h in the ischemic tissue relative to the uninjured control limb and was attributed to the higher concentration of ROS in the ischemic tissue.
CONCLUSIONS: These results demonstrate the potential for non-invasive sensing for interrogating the inflammatory and healing response in ischemic tissue.
Selena Magalotti; Tiffany P Gustafson; Qian Cao; Dana R Abendschein; Richard A Pierce; Mikhail Y Berezin; Walter J Akers
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular imaging and biology : MIB : the official publication of the Academy of Molecular Imaging     Volume:  15     ISSN:  1860-2002     ISO Abbreviation:  Mol Imaging Biol     Publication Date:  2013 Aug 
Date Detail:
Created Date:  2013-07-11     Completed Date:  2014-02-11     Revised Date:  2014-03-19    
Medline Journal Info:
Nlm Unique ID:  101125610     Medline TA:  Mol Imaging Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  423-30     Citation Subset:  IM    
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MeSH Terms
Biosensing Techniques / methods*
Disease Models, Animal
Electrophoresis, Polyacrylamide Gel
Fluorescent Dyes / chemical synthesis,  chemistry,  diagnostic use*
Hindlimb / blood supply*,  pathology
Inflammation / diagnosis*,  pathology
Ischemia / diagnosis*,  pathology
Mice, Inbred C57BL
Muscles / pathology
Reactive Oxygen Species / diagnostic use*
Spectroscopy, Near-Infrared*
Tissue Distribution
Tyrosine / analogs & derivatives,  metabolism
Grant Support
HHSN268201000046C/HB/NHLBI NIH HHS; HHSN268201000046C//PHS HHS; K01 OD010935/OD/NIH HHS; K01RR026095/RR/NCRR NIH HHS; P30 CA091842/CA/NCI NIH HHS; P50 HL084922/HL/NHLBI NIH HHS; P50 HL084922/HL/NHLBI NIH HHS; R21 CA149814/CA/NCI NIH HHS; R21CA149814/CA/NCI NIH HHS
Reg. No./Substance:
0/Fluorescent Dyes; 0/Reactive Oxygen Species; 3604-79-3/3-nitrotyrosine; 42HK56048U/Tyrosine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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