| Evaluation of IgG RAST FEIA for the assay of venom-specific IgG antibodies during venom immunotherapy. | |
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MedLine Citation:
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PMID: 9751847 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Successful venom immunotherapy (VIT) in Hymenoptera allergy is usually associated with a strong increase in venom-specific serum IgG antibodies (sIgG). METHODS: We evaluated a new commercial test for the assay of sIgG (Pharmacia CAP Systemtrade mark IgG RAST(R) FEIA; FEIA), in comparison with a conventional ELISA technique. Sera from 40 bee- and 40 Vespula-allergic patients were analyzed by FEIA and ELISA before and 3 months after starting VIT. RESULTS: The correlation between sIgG obtained with the two methods was significant: r = 0.862, p<0.0001 for bee venom (BV), r = 0.861, p<0.0001 for Vespula venom (VV). The geometric mean values obtained with FEIA were higher for both venoms (BV p = 0.03; VV p<0. 01). A highly significant increase (p<0.0001) was observed during VIT with both methods. This increase was concordant in 93% of VV- and 90% of BV-treated patients. Intra- and interassay relative coefficients of variation were below 10% for FEIA. CONCLUSION: IgG RAST FEIA is a reproducible and sensitive method for the assay of venom-specific sIgG. |
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Authors:
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P Meier; U Müller |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: International archives of allergy and immunology Volume: 117 ISSN: 1018-2438 ISO Abbreviation: Int. Arch. Allergy Immunol. Publication Date: 1998 Sep |
Date Detail:
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Created Date: 1998-10-21 Completed Date: 1998-10-21 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9211652 Medline TA: Int Arch Allergy Immunol Country: SWITZERLAND |
Other Details:
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Languages: eng Pagination: 46-51 Citation Subset: IM |
Affiliation:
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Division of Internal Medicine, Zieglerspital, Bern, Switzerland. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Allergens
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immunology Antibody Specificity Bee Venoms / immunology* Desensitization, Immunologic* / standards Enzyme-Linked Immunosorbent Assay / methods, standards Humans Immunoglobulin G / blood* Radioallergosorbent Test / methods*, standards Reagent Kits, Diagnostic* Reproducibility of Results Wasp Venoms / immunology* |
| Chemical | |
Reg. No./Substance:
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0/Allergens; 0/Bee Venoms; 0/Immunoglobulin G; 0/Reagent Kits, Diagnostic; 0/Wasp Venoms |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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