| Evaluation of a IS6110-Taqman real- time PCR assay to detect Mycobacterium tuberculosis in sputum samples of patients with pulmonary TB. | |
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MedLine Citation:
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PMID: 23279625 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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AIM: Evaluate the IS6110-Taqman system performance in sputum samples from patients with pulmonary tuberculosis from health services in northeastern Brazil as a diagnostic laboratory tool for pulmonary tuberculosis. METHODS AND RESULTS: 165 sputum samples from respiratory symptomatic patients were evaluated in the IS6110-TaqMan assay: 66 patients with pulmonary tuberculosis and 99 without TB. When the IS6110-TaqMan assay was evaluated using culture and/or clinical response to the specific treatment as the gold-standard, IS6110-TaqMan assay obtained a sensitivity of 87.9% and specificity of 98%. The performance IS6110-TaqMan assay was also evaluated with the sputum smear microscopy, resulting in a sensitivity of 79.7% and specificity 94.8%. CONCLUSIONS: The IS6110-TaqMan was rapid, sensitive and specific for the diagnosis of pulmonary TB. © 2012The Authors Journal of Applied Microbiology © 2012 The Society for Applied Microbiology. |
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Authors:
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Laís Ariane de Siqueira Lira; Fabiana Cristina Fulco Santos; Márcia Schneider Zuzarte Carvalho; Rosana de Albuquerque Montenegro; Juliana Figueirêdo da Costa Lima; Haiana Charifker Schindler; Lílian Maria Lapa Montenegro |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-12-26 |
Journal Detail:
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Title: Journal of applied microbiology Volume: - ISSN: 1365-2672 ISO Abbreviation: J. Appl. Microbiol. Publication Date: 2012 Dec |
Date Detail:
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Created Date: 2013-1-2 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9706280 Medline TA: J Appl Microbiol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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© 2012The Authors Journal of Applied Microbiology © 2012 The Society for Applied Microbiology. |
Affiliation:
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Departament of Imunology, Aggeu Magalhães Research Center, Fiocruz, Recife, Pernambuco, Brazil. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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