Document Detail


Evaluation of a High-Throughput Screening Method for the Detection of the Excipient-Mediated Precipitation Inhibition of Poorly Soluble Drugs.
MedLine Citation:
PMID:  23116459     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Abstract The objective of the present study was to develop and evaluate a high-throughput (HT) precipitation inhibitor screening method that can be used for the identification of the excipient-mediated precipitation inhibition of poorly soluble drugs. The impact of incubation temperature, shaking intensity, phase separation, inter- and intraday variability, cosolvent, and plate selection on the HT screening method performance was investigated. Additionally, the pipetting quality of the automated workstation, the correlation with the classical laboratory approach, and the practical implementation of the developed HT screening method using two model compounds are disclosed. Investigation of the HT method resulted in optimized experimental conditions, which showed low inter- and intraday variability (relative standard deviation [RSD] <5.88%). Higher shaking intensity (7 Hz) and incubation temperature (37°C) resulted in a lower likelihood of obtaining false-negative results. The acceptable dimethyl sulfoxide concentration in the precipitation inhibitor screening assay was set to ≤1% (v/v). All liquid dispensing steps resulted in an RSD of <3.4%, and an excellent correlation (R(2)=0.96, P<0.01) with the classical laboratory method was obtained. The practical implementation of the developed HT method was demonstrated by investigating the impact of 23 diverse excipients on the precipitation inhibition of two poorly soluble drugs (fenofibrate and carbamazepine). The screen resulted in the identification of hit excipients, which were not identical for fenofibrate and carbamazepine. This outcome emphasized that the HT screening approach is a reasonable starting point for searching for effective precipitation inhibitors, especially because the excipient-mediated precipitation inhibition effect is case specific and cannot be predicted in a straightforward manner.
Authors:
Marija Petruševska; Uroš Urleb; Luka Peternel
Related Documents :
22980319 - Determination of bulleyaconitine a in plasma by a sensitive lc-ms/ms method and its app...
24873509 - A simple capillary electrophoresis-mass spectrometry method for complex glycan analysis...
23112269 - Validated chromatographic methods for determination of perindopril and amlodipine in ph...
24009049 - Asenapine (saphris(r)): gc-ms method validation and the postmortem distribution of a ne...
23436249 - Development of a new uplc-msms method for the determination of temozolomide in mice: ap...
19796479 - Rapid molecular imaging using attenuated total internal reflection planar array infrare...
17390619 - Nondigest liberation of biomarkers from plasma: a novel two-stage ultrafiltration appro...
3700889 - Analysis of plasma histamine: a modification of the enzymatic isotopic assay.
22138589 - Quantification of the fabry marker lysogb3 in human plasma by tandem mass spectrometry.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-1
Journal Detail:
Title:  Assay and drug development technologies     Volume:  -     ISSN:  1557-8127     ISO Abbreviation:  Assay Drug Dev Technol     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-2     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101151468     Medline TA:  Assay Drug Dev Technol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
1 Pharmaceutical and Biological Profiling, Sandoz Development Center Slovenia , Ljubljana, Slovenia .
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  A randomized phase I study of methanesulfonyl fluoride, an irreversible cholinesterase inhibitor, fo...
Next Document:  Prevalence of body-focused repetitive behaviors in three large medical colleges of Karachi: a cross-...