Document Detail

Evaluation of Fungal Chitosan as a Biocontrol and Antibacterial Agent using Fluorescence-Labeling.
MedLine Citation:
PMID:  23270832     Owner:  NLM     Status:  Publisher    
Chitosan is a precious biological polysaccharide that could be applied in several fields. Fluorescein isothiocyanate-labeled chitosan (FITC-CTS) was synthesized as a macromolecular fluorophore, added to fungal chitosan from Aspergillus niger, to investigate the interaction mechanism and antibacterial performance of (FITC-CTS) against Escherichia coli and Micrococcus leteus. Fluorescence imaging was used to demonstrate chitosan effect on the bacterial cells. Fluorescence density of treated bacteria with FITC-CTS was correlated with viable cell number. Electron micrographs of treated E. coli with fungal chitosan revealed that chitosan principally interact with bacterial cell wall, causing cell wall lyses with exposure time prolongation. Fungal chitosan could be proposed for bacterial growth control as a powerful, natural and safe alternative to synthetic and chemical bactericides. Fluorescence labeling proved to be an efficient tool for determining the antimicrobial activity of chitosan.
Shaaban H Moussa; Ahmed A Tayel; Ahmed I Al-Turki
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-12-24
Journal Detail:
Title:  International journal of biological macromolecules     Volume:  -     ISSN:  1879-0003     ISO Abbreviation:  Int. J. Biol. Macromol.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7909578     Medline TA:  Int J Biol Macromol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012. Published by Elsevier B.V.
Promising Researches Center in Biological Control and Agricultural Information, Qassim University, Bureidah 51452, Saudi Arabia; Genetic Engineering and Biotechnology Research Institute, Minoufiya University, El-Sadat City, Egypt.
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