Document Detail

Evaluation of four different equations for calculating LDL-C with eight different direct HDL-C assays.
MedLine Citation:
PMID:  23628525     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Low-density lipoprotein cholesterol (LDL-C) is often calculated (cLDL-C) by the Friedewald equation, which requires high-density lipoprotein cholesterol (HDL-C) and triglycerides (TG). Because there have been considerable changes in the measurement of HDL-C with the introduction of direct assays, several alternative equations have recently been proposed.
METHODS: We compared 4 equations (Friedewald, Vujovic, Chen, and Anandaraja) for cLDL-C, using 8 different direct HDL-C (dHDL-C) methods. LDL-C values were calculated by the 4 equations and determined by the β quantification reference method procedure in 164 subjects.
RESULTS: For normotriglyceridemic samples (TG<200mg/dl), between 6.2% and 24.8% of all results exceeded the total error goal of 12% for LDL-C, depending on the dHDL-C assay and cLDL-C equation used. Friedewald equation was found to be the optimum equation for most but not all dHDL-C assays, typically leading to less than 10% misclassification of cardiovascular risk based on LDL-C. Hypertriglyceridemic samples (>200mg/dl) showed a large cardiovascular risk misclassification rate (30%-50%) for all combinations of dHDL-C assays and cLDL-C equations.
CONCLUSION: The Friedewald equation showed the best performance for estimating LDL-C, but its accuracy varied considerably depending on the specific dHDL-C assay used. None of the cLDL-C equations performed adequately for hypertriglyceridemic samples.
Marcelo Jose Andrade Oliveira; Hendrick E van Deventer; Lorin M Bachmann; G Russell Warnick; Katsuyuki Nakajima; Masakasu Nakamura; Ikunosuke Sakurabayashi; Mary M Kimberly; Robert D Shamburek; William J Korzun; Gary L Myers; W Greg Miller; Alan T Remaley
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, N.I.H., Intramural; Research Support, Non-U.S. Gov't     Date:  2013-04-27
Journal Detail:
Title:  Clinica chimica acta; international journal of clinical chemistry     Volume:  423     ISSN:  1873-3492     ISO Abbreviation:  Clin. Chim. Acta     Publication Date:  2013 Aug 
Date Detail:
Created Date:  2013-06-03     Completed Date:  2014-01-07     Revised Date:  2014-09-21    
Medline Journal Info:
Nlm Unique ID:  1302422     Medline TA:  Clin Chim Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  135-40     Citation Subset:  IM    
Copyright Information:
Published by Elsevier B.V.
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MeSH Terms
Biological Assay / standards*
Blood Chemical Analysis / methods*,  standards*
Cardiovascular Diseases / blood,  diagnosis
Cholesterol, HDL / blood*
Cholesterol, LDL / blood*
Grant Support
Z01 CL010303-07/CL/CLC NIH HHS
Reg. No./Substance:
0/Cholesterol, HDL; 0/Cholesterol, LDL

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