Document Detail


Evaluation of enzyme immunoassays to detect Clostridium difficile toxin from anaerobic stool culture.
MedLine Citation:
PMID:  19095569     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Stool culture for Clostridium difficile, while necessary for strain typing and antimicrobial surveillance, cannot determine toxin production. We prospectively tested in triplicate 91 C difficile cultured isolates for toxin production by 2 enzyme immunoassays (EIAs) (Meridian Premier Toxins A&B, Meridian Bioscience, Cincinnati, OH; and TechLab Tox A/B II, TechLab, Blacksburg, VA) and cytotoxin neutralization bioassay (CTN). By CTN, 88% (80/91) were toxigenic. Reproducibility was 93% (85/91) for CTN, 80% (73/91) for Meridian EIA, and 79% (72/91) for TechLab EIA. Compared with CTN, sensitivities were 87.1% and 89.2% for the Meridian and TechLab EIAs, respectively. In an additional 115 stool specimens, CTN detected toxin more frequently from cultured isolates (96/115) than stool (84/115). For C difficile toxin detection from isolates, EIA was less reproducible than CTN. EIA methods can be falsely negative in 10% to 12% of isolates, and these should be tested by CTN or polymerase chain reaction. When positive, EIA is fast and reliable for detecting C difficile toxin from culture.
Authors:
Rosemary C She; Robert J Durrant; Cathy A Petti
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Publication Detail:
Type:  Comparative Study; Evaluation Studies; Journal Article    
Journal Detail:
Title:  American journal of clinical pathology     Volume:  131     ISSN:  1943-7722     ISO Abbreviation:  Am. J. Clin. Pathol.     Publication Date:  2009 Jan 
Date Detail:
Created Date:  2008-12-19     Completed Date:  2009-01-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370470     Medline TA:  Am J Clin Pathol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  81-4     Citation Subset:  AIM; IM    
Affiliation:
Department of Pathology, University of Utah School of Medicine, Salt Lake City, UT, USA.
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MeSH Terms
Descriptor/Qualifier:
Anaerobiosis
Clostridium difficile / immunology*
Cytotoxins / antagonists & inhibitors
Enterotoxins / analysis*
Feces / chemistry*,  microbiology
Humans
Immunoenzyme Techniques / methods
Predictive Value of Tests
Prospective Studies
Reproducibility of Results
Sensitivity and Specificity
Chemical
Reg. No./Substance:
0/Cytotoxins; 0/Enterotoxins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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