Document Detail

Evaluation of the Effect of PEGylated Single-Walled Carbon Nanotubes on Viability and Proliferation of Jurkat Cells.
MedLine Citation:
PMID:  25317182     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Among the numerous nanosized drug delivery systems currently under investigation, carbon nanotubes (CNTs), regardless of being single or multiple-walled, offer several advantages and are considered as promising candidates for drug targeting. Despite the valuable potentials of CNTs in drug delivery, their toxicity still remains an important issue. After the PEGylation of single-walled CNTs (SWCNTs) with phospholipid-PEG (Pl-PEG) conjugates to prepare water-dispersible nanostructures, the present study was designed to evaluate whether the functionalization with Pl-PEG derivatives could alter the cytotoxic response of cells in culture, affect their viability and proliferation. In-vitro cytotoxicity screens were performed on cultured Jurkat cells. The SWCNTs samples used in this exposure were pristine SWCNTs, Pl-PEG 2000/5000-SWCNTs at various concentrations. Jurkat cells were first incubated for 3 h at 37°C with test materials and seeded in 6-well culture plates at a given concentration. The plates were then incubated for 24, 48 and 72 h at 37°C in a 5% CO2 humidified incubator. Cell Viability and proliferation assay were performed using trypan blue exclusion test and the cell cycle kinetic status of Jurkat cells was analyzed by flow cytometry. Cell morphology was finally studied using double staining technique and a fluorescence microscope. We found that, regardless of the duration of exposure, functionalized SWCNTs were substantially less toxic, compared to pure SWCNTs and that the molecular weight of Pl-PEGs played an important role at higher concentrations. In conclusion, our noncovalent protocol seemed to be effective for increasing SWCNTs biocompatibility.
Naghmeh Hadidi; Seyed Farshad Hosseini Shirazi; Farzad Kobarfard; Nastaran Nafissi-Varchehd; Reza Aboofazeli
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Iranian journal of pharmaceutical research : IJPR     Volume:  11     ISSN:  1735-0328     ISO Abbreviation:  Iran J Pharm Res     Publication Date:  2012  
Date Detail:
Created Date:  2014-10-15     Completed Date:  2014-10-15     Revised Date:  2014-10-23    
Medline Journal Info:
Nlm Unique ID:  101208407     Medline TA:  Iran J Pharm Res     Country:  Iran    
Other Details:
Languages:  eng     Pagination:  27-37     Citation Subset:  -    
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