| Evaluation of Developmental Competence of In Vitro-produced Porcine Embryos Based on the Timing, Pattern and Evenness of the First Cleavage and Onset of the Second Cleavage. | |
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MedLine Citation:
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PMID: 20657154 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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The following selection markers for in vitro-produced porcine embryos were investigated: the timing, pattern and evenness of the first cleavage and the timing of the second cleavage. The embryos that cleaved by 30 h post-insemination (hpi) developed to blastocysts at a significantly higher rate (60.9%) and with a significantly higher cell number (33.6 cells) than those of embryos cleaved by 36 hpi (26.4% and 23.6 cells, respectively, P<0.05). Blastocyst proportions derived from 2- and 3-cell embryos cleaved by 30 hpi (68.2 and 65.3%, respectively) were significantly higher than those of 4- and >4-cell embryos (46.3 and 42.6%, respectively, P<0.05). The cell number per blastocyst generated from 2-cell embryos was significantly greater (37.3 cells) than those from 3-, 4- and >4-cell embryos (23.6-27.8 cells, P<0.05). Among embryos cleaved by 30 hpi, the blastocysts derived from evenly cleaved embryos (40.6 cells) were of significantly better quality than those derived from unevenly cleaved embryos (33.2 cells, P<0.05), although their blastocyst rates did not differ. The evenly cleaved embryos that underwent subsequent cleavage within 18 h had significantly higher blastocyst rates (72.7-81.0%) and quality (36.2-40.9 cells) than those without subsequent cleavage (48.3% and 22.5 cells, respectively, P<0.05) during the same period. In conclusion, the timing, pattern and evenness of the first cleavage and the timing of the second cleavage affected the developmental competence and quality of in vitro-produced porcine embryos. |
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Authors:
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Thanh Quang Dang-Nguyen; Kazuhiro Kikuchi; Tamas Somfai; Manabu Ozawa; Michiko Nakai; Naoki Maedomari; Nguyen Viet-Linh; Yukio Kanai; Bui Xuan Nguyen; Takashi Nagai |
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Publication Detail:
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Type: Journal Article Date: 2010-07-20 |
Journal Detail:
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Title: The Journal of reproduction and development Volume: 56 ISSN: 0916-8818 ISO Abbreviation: J. Reprod. Dev. Publication Date: 2011 Dec |
Date Detail:
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Created Date: 2011-01-19 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9438792 Medline TA: J Reprod Dev Country: Japan |
Other Details:
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Languages: eng Pagination: 593-600 Citation Subset: IM |
Affiliation:
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Department of Animal Science, National Institute of Agrobiological Sciences. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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