Document Detail

Evaluation of DNA probe removal from nylon membrane.
MedLine Citation:
PMID:  1362069     Owner:  NLM     Status:  MEDLINE    
Genetic fingerprinting is one of the most challenging applications of any hybridization membranes. Forensic DNA fingerprinting typically uses samples in the range of 100-400 ng of genomic DNA. To ensure the ability to successfully reprobe the samples, it is imperative that repeated stripping of sample DNA be minimized while stripping of the probe DNA be maximized. By using standard dilutions of K562 cell line, we compared the following three stripping techniques: NaOH at 25 degrees C, formamide (HCONH2) at 65 degrees C and 0.1 x standard saline citrate and 0.1% sodium dodecyl sulfate at 95 degrees C (high-temperature stripping). The largest amount of genomic DNA was stripped from the membrane with NaOH, with the other two techniques removing less. Formamide and high-temperature procedures resulted in a loss of approximately 5-10 ng of DNA per strip. In contrast, the NaOH resulted in a loss of approximately 10-20 ng per strip.
K Noppinger; G Duncan; D Ferraro; S Watson; J Ban
Related Documents :
17119629 - Cross-species investigation of helicoverpa armigera microsatellites as potential marker...
14532179 - Madurella mycetomatis strains from mycetoma lesions in sudanese patients are clonal.
21726129 - Inhibition of hiv-1 reverse transcriptase associated activities by the hydroalcoholic e...
21709779 - Hpv pseudovirions as dna delivery vehicles.
22237529 - Pcr amplification, cloning, and sequencing of ancient dna.
21782619 - Cytochrome p450 (cyp1a) induction and dna adducts in a rat hepatoma cell line (fao), ex...
8439949 - Benzene and its phenolic metabolites produce oxidative dna damage in hl60 cells in vitr...
22569599 - Validation of methylation-sensitive high resolution melting for the detection of dna me...
17630719 - Escherichia coli genosensor based on polyaniline.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  BioTechniques     Volume:  13     ISSN:  0736-6205     ISO Abbreviation:  BioTechniques     Publication Date:  1992 Oct 
Date Detail:
Created Date:  1993-02-08     Completed Date:  1993-02-08     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8306785     Medline TA:  Biotechniques     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  572-5     Citation Subset:  IM    
Broward County Sheriff's Crime Laboratory, DNA Unit, Ft. Lauderdale, FL 33301.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
DNA Fingerprinting / methods*
DNA Probes / isolation & purification*
Evaluation Studies as Topic
Hot Temperature
Membranes, Artificial
Nucleic Acid Hybridization
Polymorphism, Restriction Fragment Length
Sodium Hydroxide
Reg. No./Substance:
0/DNA Probes; 0/Formamides; 0/Membranes, Artificial; 0/Nylons; 1310-73-2/Sodium Hydroxide; 75-12-7/formamide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Preparation of plasmid DNA by sequential enzymatic digestion.
Next Document:  Pulmonary artery involvement in Takayasu arteritis. A case of right ventricular failure as presentat...