Document Detail


Evaluation of the DNA damaging effects of amitraz on human lymphocytes in the Comet assay.
MedLine Citation:
PMID:  23385813     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Amitraz is formamidine pesticide widely used as insecticide and acaricide. In veterinary medicine, amitraz has important uses against ticks, mites and lice on animals. Also, amitraz is used in apiculture to control Varroa destructor. It this study, the alkaline Comet assay was used to evaluate DNA damaging effects of amitraz in human lymphocytes. Isolated human lymphocytes were incubated with varying concentrations of amitraz (0.035, 0.35, 3.5, 35 and 350 mu g/mL). The Comet assay demonstrated that all concentrations of amitraz caused statistically significant increase in the level of DNA damage, thus indicating that amitraz possesses genotoxic potential. The concentration of amitraz that produced the highest DNA damage (3.5 mu g/mL) was chosen for further analysis with the antioxidant catalase. The obtained results showed that co-treatment with antioxidant catalase (100 IU/mL or 500 IU/mL) significantly reduced the level of DNA damage, indicating the possible involvement of reactive oxygen species in DNA damaging effects of amitraz. Flow cytometric analysis revealed increase of the apoptotic index following treatment with amitraz. However, co-treatment with catalase reduced the apoptotic index, while treatment with catalase alone reduced the percentage of apoptotoc cells even in comparison with the negative control. Therefore, catalase had protective effects against ROS-mediated DNA damage and apoptosis.
Authors:
Milena Radakovic; Jevrosima Stevanovic; Ninoslav Djelic; Nada Lakic; Jelena Knezevic-Vukcevic; Branka Vukovic-Gacic; Zoran Stanimirovic
Related Documents :
11524013 - Diepoxybutane and diepoxyoctane interstrand cross-linking of the 5s dna nucleosomal cor...
8487123 - Immunological probing of induction and repair of 8-methoxypsoralen photoadducts in dna ...
24271193 - Assembly of nucleosome-like structures mediated by cauliflower dna topoisomerase.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of biosciences     Volume:  38     ISSN:  0973-7138     ISO Abbreviation:  J. Biosci.     Publication Date:  2013 Mar 
Date Detail:
Created Date:  2013-02-06     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8100809     Medline TA:  J Biosci     Country:  India    
Other Details:
Languages:  eng     Pagination:  53-62     Citation Subset:  IM    
Affiliation:
Department of Biology, Faculty of Veterinary Medicine, Faculty of Biology, The University of Belgrade, Belgrade, Serbia.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Suppressors of RNA silencing encoded by tomato leaf curl betasatellites.
Next Document:  Fatty acyl chain-dependent but charge-independent association of the SH4 domain of Lck with lipid me...