Document Detail

Evaluation of CellSolutions BestPrep® Automated Thin-Layer Liquid-Based Cytology Papanicolaou Slide Preparation and BestCyte® Cell Sorter Imaging System.
MedLine Citation:
PMID:  25277345     Owner:  NLM     Status:  Publisher    
Objective: A double-blind study was conducted to compare the performance of the new BestPrep® (CellSolutions) liquid-based thin-layer Papanicolaou (Pap) test with ThinPrep® (Hologic). Study Design: Samples from the study patients (n = 105) were collected twice in the same encounter with the ThinPrep sample always taken first and the BestPrep sample collected second. Slides were prepared according to both manufacturers' protocols and evaluated using manual microscopic review and the BestCyte® cell sorter imaging system (CellSolutions). Diagnostic truth for each case was determined by independent manual review of both slides by multiple pathologists and histology when available. The presence of atypical squamous cells of undetermined significance was the threshold for positive for sensitivity and specificity calculations. Results: BestPrep and ThinPrep, by manual review, had sensitivities for high-grade squamous intraepithelial lesion (HSIL) cases of 100 and 95.6%, respectively. Using the BestCyte cell sorter, both had 100% sensitivity. For the same HSIL cases, the digene HC2 high-risk human papillomavirus DNA test had sensitivities of 100% (BestPrep) and 95.6% (ThinPrep). Specificities were 71.4% (BestPrep) and 54.8% (ThinPrep). Conclusions: BestPrep was equivalent to ThinPrep for manual review even though BestPrep was always the second sample collected. The BestCyte cell sorter provides a practical alternative to manual review for both BestPrep and ThinPrep slides. © 2014 S. Karger AG, Basel.
Agnes Delga; Frederic Goffin; Frederic Kridelka; Raphaël Marée; Chantal Lambert; Philippe Delvenne
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-9-27
Journal Detail:
Title:  Acta cytologica     Volume:  -     ISSN:  0001-5547     ISO Abbreviation:  Acta Cytol.     Publication Date:  2014 Sep 
Date Detail:
Created Date:  2014-10-3     Completed Date:  -     Revised Date:  2014-10-4    
Medline Journal Info:
Nlm Unique ID:  0370307     Medline TA:  Acta Cytol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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