Document Detail


Evaluation of bovine embryo biopsy techniques according to their ability to preserve embryo viability.
MedLine Citation:
PMID:  23091350     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The purpose of this research was to evaluate three embryo biopsy techniques used for preimplantation genetic diagnosis (PGD) in cattle and to recommend the least invasive one for current use, especially when PGD is followed by embryo cryopreservation. Three hundred bovine embryos were biopsied by either one of the needle, aspiration or microblade method, and then checked for viability by freezing/thawing and transplantation to recipient cows. The number of pregnancies obtained after the transfer of biopsied frozen/thawed embryos was assessed 30 days later using ultrasounds. The results were significantly different between the three biopsy methods: the pregnancy rate was of 57% in cows that received embryos biopsied by needle, 43% in cows that received embryos biopsied by aspiration, and 31% in cows that received embryos biopsied by microblade. Choosing an adequate biopsy method is therefore of great importance in embryos that will undergo subsequent cryopreservation, as it significantly influences their viability after thawing.
Authors:
M Cenariu; E Pall; C Cernea; I Groza
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-10-02
Journal Detail:
Title:  Journal of biomedicine & biotechnology     Volume:  2012     ISSN:  1110-7251     ISO Abbreviation:  J. Biomed. Biotechnol.     Publication Date:  2012  
Date Detail:
Created Date:  2012-10-23     Completed Date:  2013-01-16     Revised Date:  2013-07-11    
Medline Journal Info:
Nlm Unique ID:  101135740     Medline TA:  J Biomed Biotechnol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  541384     Citation Subset:  IM    
Affiliation:
Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, 3-5 Calea Manastur, 400372 Cluj-Napoca, Romania. mihai.cenariu@usamvcluj.ro
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MeSH Terms
Descriptor/Qualifier:
Animals
Biopsy / methods*
Cattle
Embryo, Mammalian / pathology*,  physiology*
Female
Pregnancy
Pregnancy Outcome*
Preimplantation Diagnosis / methods*
Tissue Survival / physiology*
Comments/Corrections

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