Document Detail


Evaluation of BC-3 and BCP-1 cell lines in immunofluorescence assay to detect HHV-8 antibodies in Kaposi's sarcoma, multiple myeloma and other groups.
MedLine Citation:
PMID:  10382819     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: We describe a comparative study of an immunofluorescence assay using inducible BC-3 and BCP-1 cell lines as sources of HHV-8 antigens. STUDY DESIGN: Detection of both antibodies to proteins expressed in lytic cycle and during latency in sera from HIV-infected patients with KS, HIV-positive patients without KS, normal blood donors, HIV-negative pregnant women and HIV-negative patients with multiple myeloma. Where possible, detection of antibody was associated with nested PCR detection of HHV-8 in peripheral mononuclear cell (PBMC) samples collected from AIDS-KS patients. RESULTS: Immunofluorescence was more intense with the BC-3 cell line than with BCP-1, thus facilitating examination under the microscope. HHV-8 antibodies were detected among 82.75% of AIDS-KS patients, in 27.3% of HIV-infected homosexual men, 2% of blood donors and in 2% of pregnant women. No HHV-8 antibodies were detected in serum samples from HIV-negative patients presenting multiple myeloma. HHV-8 DNA sequences were detected and confirmed by southern blot hybridization in five out of 17 (29.4%) PBMC samples from AIDS-KS patients. Titre of antibodies to proteins expressed in lytic cycle was much higher than the titre of antibodies to proteins expressed during latency. CONCLUSIONS: Both immunofluorescence assays were found useful and HHV-8 seroprevalence rates reported in previous studies were confirmed. In addition, results obtained using these assays tend to provide evidence for a lack of epidemiological association between HHV-8 infection and development of multiple myeloma.
Authors:
J P Couty; S Ranger-Rogez; C Venot; A Jaccard; J P Rogez; P Weinbreck; D Bordessoule; F Denis
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology     Volume:  12     ISSN:  1386-6532     ISO Abbreviation:  J. Clin. Virol.     Publication Date:  1999 May 
Date Detail:
Created Date:  1999-08-26     Completed Date:  1999-08-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9815671     Medline TA:  J Clin Virol     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  221-31     Citation Subset:  IM; X    
Affiliation:
Department of Microbiology, EP CNRS 118, Limoges, France. j.couty@lemel.fr
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MeSH Terms
Descriptor/Qualifier:
Adult
Antibodies, Viral / blood,  immunology*
Cell Line
Cross Reactions
Epstein-Barr Virus Nuclear Antigens / immunology
Female
Fluorescent Antibody Technique
HIV Infections / complications
Herpesvirus 4, Human / immunology
Herpesvirus 8, Human / genetics,  immunology*
Humans
Male
Middle Aged
Multiple Myeloma / immunology*,  virology
Polymerase Chain Reaction
Pregnancy
Sarcoma, Kaposi / immunology*,  virology
Staining and Labeling / methods
Chemical
Reg. No./Substance:
0/Antibodies, Viral; 0/Epstein-Barr Virus Nuclear Antigens

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