Document Detail


Evaluation of antifungal efficacy of erbium, chromium: yttrium-scandium-gallium-garnet laser against Candida albicans.
MedLine Citation:
PMID:  20666577     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: The purpose of this in vitro study was to investigate the ability of Er,Cr:YSGG laser irradiation at different energy settings to eliminate Candida albicans, either with or without 5.25% sodium hypochlorite (NaOCl) treatment.
METHODS: In total, 90 extracted human single-rooted teeth were shaped by using a crown-down technique with HERO Shaper rotary instruments and irrigated with 1 mL of 2.5% NaOCl between each instrument. The specimens were autoclaved and incubated with a suspension of C. albicans (ATCC 90028). Then the specimens were divided into six treatment groups (n = 15 per group): group 1, NaOCl + 1-W laser; group 2, 1-W laser; group 3, NaOCl + 0.75-W laser; group 4, 0.75-W laser, group 5, NaOCl; and group 6, no treatment. Aliquots of the experimental teeth were transferred onto Sabouraud's dextrose agar plates with paper points, and colony-forming units (CFUs) were counted as a measure of antifungal activity. One root from each group, which was not submitted to the sampling procedure, was fixed in glutaraldehyde, split into two halves, and evaluated with scanning electron microscopy (SEM). Statistical analysis was performed by using analysis of variance (ANOVA) and the Dunnett t test. Significance was set at alpha = 0.05.
RESULTS: All the treatment groups resulted in a significant reduction of C. albicans, but no sterilization, and all the groups were significantly different from each other (p < 0.0001). Group 1 (NaOCl and 1-W laser combination) exhibited the greatest reduction, whereas group 5 (NaOCl) resulted in a minimal reduction in CFUs of C. albicans. The SEM observations were in accordance with the microbiologic analysis.
CONCLUSIONS: The 5.25% NaOCl and 1-W laser treatment combination may be a valuable cleaning tool for root canals infected with C. albicans.
Authors:
Emel Olga Onay; Ceren Alikaya; Esra Seker
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Photomedicine and laser surgery     Volume:  28 Suppl 1     ISSN:  1557-8550     ISO Abbreviation:  Photomed Laser Surg     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-08-09     Completed Date:  2010-12-16     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101222340     Medline TA:  Photomed Laser Surg     Country:  United States    
Other Details:
Languages:  eng     Pagination:  S73-8     Citation Subset:  IM    
Affiliation:
Department of Endodontics, Baskent University, School of Dentistry, Ankara, Turkey. eonay@baskent.edu.tr
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MeSH Terms
Descriptor/Qualifier:
Candida albicans / radiation effects*
Dental Disinfectants / therapeutic use
Dental Pulp Cavity / microbiology*,  ultrastructure
Dose-Response Relationship, Radiation
Humans
Lasers, Solid-State* / therapeutic use
Microscopy, Electron, Scanning
Sodium Hypochlorite / therapeutic use
Stem Cells
Chemical
Reg. No./Substance:
0/Dental Disinfectants; 7681-52-9/Sodium Hypochlorite

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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