Document Detail

Evaluation of the 3 alpha-hydroxysteroid dehydrogenase assay for ursodeoxycholic acid, and 7 oxo- and 12 oxo- bile acids.
MedLine Citation:
PMID:  6929859     Owner:  NLM     Status:  MEDLINE    
3 alpha-Hydroxysteroid dehydrogenase (SDH) quantifies ursodeoxycholic acid, 3 alpha,12 alpha-diol-7-one-5 beta-cholanoic acid, 3 alpha-ol-7-one-5 beta-cholanoic acid, and 3 alpha,7 alpha-diol-12-one-5 beta-cholanoic acid in a manner similar to the more commonly measured bile acids, exemplified by taurocholic acid. The type of oxygen function at the 7 or 12 position and its orientation at the 7 position has no effect on the rate of reaction of the enzyme. Discrepancies in the glycine/taurine ratios of patient intestinal aspirates containing ursodeoxycholic acid and oxo bile acids, obtained by SDH and gas-liquid chromatographic methods are not a result of the enzyme assay procedure.
L J Haeffner; S J Gordon; J S Magen; O D Kowlessar
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Journal of lipid research     Volume:  21     ISSN:  0022-2275     ISO Abbreviation:  J. Lipid Res.     Publication Date:  1980 May 
Date Detail:
Created Date:  1980-08-28     Completed Date:  1980-08-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0376606     Medline TA:  J Lipid Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  477-80     Citation Subset:  IM    
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MeSH Terms
3-Hydroxysteroid Dehydrogenases*
Bile / analysis
Bile Acids and Salts / analysis
Chenodeoxycholic Acid / analogs & derivatives,  analysis
Cholic Acids / analysis*
Chromatography, Gas
Deoxycholic Acid / analogs & derivatives*,  analysis
Glycine / analysis
Intestines / analysis
Lithocholic Acid / analogs & derivatives,  analysis
Taurine / analysis
Ursodeoxycholic Acid / analysis*
Reg. No./Substance:
0/Bile Acids and Salts; 0/Cholic Acids; 107-35-7/Taurine; 128-13-2/Ursodeoxycholic Acid; 2458-08-4/3,7-dihydroxy-12-oxocholanoic acid; 434-13-9/Lithocholic Acid; 4651-67-6/7-ketolithocholic acid; 474-25-9/Chenodeoxycholic Acid; 56-40-6/Glycine; 83-44-3/Deoxycholic Acid; 911-40-0/7-ketodeoxycholic acid; EC 1.1.-/3-Hydroxysteroid Dehydrogenases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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