Document Detail

Evaluation of 2,5-hexanedione in urine of workers exposed to n-hexane in Brazilian shoe factories.
MedLine Citation:
PMID:  12376131     Owner:  NLM     Status:  MEDLINE    
Urinary 2,5-hexanedione (2,5-HD) is used as a biomarker for biological monitoring of workers exposed to n-hexane. The purpose of this study was to compare two types of treatment of urine samples during clean-up (with and without acidic hydrolysis) and to study the exposure situation of workers exposed to n-hexane during shoe manufacturing. There, various glues containing n-hexane are used. Quantification of 2,5-HD was carried out by gas chromatography and flame ionization detection (GC-FID). Fifty-two urine samples taken from workers of seven shoe factories were analyzed. Thirty-four persons from the administrative staff of the same factories served as controls. They were not known to be exposed to n-hexane. The samples treated with acidic hydrolysis showed levels (average 0.94 mg/l) approximately 10 times higher than samples without acidic hydrolysis (0.09 mg/l). The difference is predominantly caused by the conversion of other metabolites of n-hexane (e.g. 4,5-dihydroxy-2-hexanone) to 2,5-HD in the presence of acids. Our results also show, that exposure to n-hexane is different between various industries. Levels of 2,5-HD in urine are predominantly dependent on the type of operation (how the glue is applied on the leather during shoe manufacturing). Simple measures, e.g. using a glue handgun instead of a paintbrush significantly decreased exposure to n-hexane.
Claudia Regina dos Santos; Miriam Meyer Meyer Passarelli; Elizabeth de Souza Nascimento
Publication Detail:
Type:  Evaluation Studies; Journal Article    
Journal Detail:
Title:  Journal of chromatography. B, Analytical technologies in the biomedical and life sciences     Volume:  778     ISSN:  1570-0232     ISO Abbreviation:  J. Chromatogr. B Analyt. Technol. Biomed. Life Sci.     Publication Date:  2002 Oct 
Date Detail:
Created Date:  2002-10-11     Completed Date:  2003-04-08     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  101139554     Medline TA:  J Chromatogr B Analyt Technol Biomed Life Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  237-44     Citation Subset:  IM    
Copyright Information:
Copyright 2002 Elsevier Science B.V.
Department of Clinical and Toxicological Analysis, Faculty of Pharmaceutical Sciences, University of São Paulo, Av Prof Lineu Prestes, 580, 05508-900 São Paulo, Brazil.
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MeSH Terms
Chromatography, Gas / methods
Hexanes / administration & dosage*
Hexanones / urine*
Occupational Exposure*
Quality Control
Sensitivity and Specificity
Reg. No./Substance:
0/Hexanes; 0/Hexanones; 110-13-4/2,5-hexanedione; 110-54-3/n-hexane

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