Document Detail


Evaluation of 1p losses in primary carcinomas, local recurrences and peripheral metastases from colorectal cancer patients.
MedLine Citation:
PMID:  11228544     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cytogenetic and molecular genetic analyses of colorectal adenomas and carcinomas have shown that loss of the distal part of chromosome arm 1p is common, particularly in tumors of the left colon. Because the importance of 1p loss in colorectal cancer metastases is unknown, we compared the frequency, exact site and extent of 1p deletions in primary carcinomas (n=28), local recurrences (n=19) and metastases (n=33) from 67 colorectal cancer patients using 14 markers in an allelic imbalance study. Loss of 1p was found in 50% of the primary carcinomas, 33% of the local recurrences, and 64% of the metastases, revealing a significant difference between the local recurrences and the metastases (P=.04). The smallest region of 1p deletion overlap (SRO) defined separately for each group of lesions had the region between markers D1S2647 and D1S2644, at 1p35-36, in common. The genes PLA2G2A (1p35.1-36) and TP73 (1p36.3) were shown to lie outside this consistently lost region, suggesting that neither of them are targets for the 1p loss. In the second part of the study, microdissected primary carcinomas and distant metastases from the same colorectal cancer patients (n=18) were analyzed, and the same 1p genotype was found in the majority of patients (12/18, 67%). The finding that primary carcinoma cells with metastatic ability usually contain 1p deletions, and that some cases lacking 1p alterations in the primary tumor acquire such changes during growth of a metastatic lesion, supports the notion that 1p loss may be important both early and late in colorectal carcinogenesis, with the apparent exception of local recurrences.
Authors:
L Thorstensen; H Qvist; S Heim; G J Liefers; J M Nesland; K E Giercksky; R A Lothe
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Neoplasia (New York, N.Y.)     Volume:  2     ISSN:  1522-8002     ISO Abbreviation:  Neoplasia     Publication Date:    2000 Nov-Dec
Date Detail:
Created Date:  2001-03-06     Completed Date:  2002-04-01     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  100886622     Medline TA:  Neoplasia     Country:  United States    
Other Details:
Languages:  eng     Pagination:  514-22     Citation Subset:  IM    
Affiliation:
Department of Genetics, Institute for Cancer Research, The Norwegian Radium Hospital, Oslo, Norway.
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MeSH Terms
Descriptor/Qualifier:
Adenocarcinoma / genetics*,  secondary
Adolescent
Adult
Aged
Aged, 80 and over
Chromosome Deletion*
Chromosomes, Human, Pair 1 / genetics*
Colorectal Neoplasms / genetics*,  pathology
DNA, Neoplasm / isolation & purification
Female
Humans
Liver Neoplasms / genetics,  secondary
Loss of Heterozygosity
Lung Neoplasms / genetics,  secondary
Male
Microsatellite Repeats
Middle Aged
Neoplasm Recurrence, Local / genetics*,  pathology
Peritoneal Neoplasms / genetics,  secondary
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Chemical
Reg. No./Substance:
0/DNA, Neoplasm
Comments/Corrections

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