| Evaluation of (111)In-labeled macrocyclic chelator-amino acid derivatives for cancer imaging. | |
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MedLine Citation:
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PMID: 22136890 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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PURPOSE: We evaluated new (111)In-labeled amino acid derivatives, in which the amino acids are conjugated with1,4,7,10-tetra-azacyclododecane-1,4,7,10-tetraacetic acid (DOTA), 1,4,7,10-tetraazacyclododecane-1,7-diacetic acid (DO2A) or 1,4,7,10-tetraazacyclododecane-1,4,7-triacetic acid (DO3A). METHODS: DOTA-aminoalanine (DOTA-A), DOTA-aminohomoalanine (DOTA-H), DOTA-lysine (DOTA-L), DO2A-alanine (DO2A-A), DO3A-alanine (DO3A-A) and DO3A-homoalanine (DO3A-H) were labeled with (111)In. In vitro cell uptake assays were performed usingHep3B (a human hepatoma cell line), CT26 (a mouse colon cancer cell line) and U87MG (a human glioma cell line). In vitro cell uptake inhibition assays were performed using U87MG and (111)In-DO3A-H. U87MG bearing xenografted mice were subject to biodistribution, SPECT imaging, autoradiography, and immunohistochemistry studies. RESULTS: Of the amino acid derivatives and cell lines examined, U87MG and (111)In-DO3A-H showed highest uptake in vitro. This uptake was blocked by 2-aminobicyclo-[2,2,1] heptane-2-carboxylic acid (BCH) and by tryptophan. (111)In-DO3A-HSPECT imaging of U87MG bearing xenografted mice visualized tumors (mean tumor-to-muscle ratio 3.16±0.74). Autoradiography and immunohistochemistry revealed that (111)In-DO3A-H uptake matched L-type amino acid transporter 1 expression. CONCLUSION: Tumor uptake was successfully imaged using (111)In-DO3A-H in U87MG bearing xenografted mice. (111)In-DO3A-H appears to be useful for imaging tumors expressing L-type amino acid transporter. |
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Authors:
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Jong Jin Lee; Dinesh Shetty; Yun-Sang Lee; Sang Eun Kim; Young Joo Kim; Mi Kyung Hong; Ji Yeon Son; Jae Min Jeong |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-11-30 |
Journal Detail:
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Title: Nuclear medicine and biology Volume: - ISSN: 1872-9614 ISO Abbreviation: - Publication Date: 2011 Nov |
Date Detail:
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Created Date: 2011-12-5 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9304420 Medline TA: Nucl Med Biol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011 Elsevier Inc. All rights reserved. |
Affiliation:
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Department of Nuclear Medicine, Radiation Applied Life Science, Institute of Radiation Medicine, Cancer Research Institute, Seoul National University College of Medicine, Seoul National University, Seoul 110-744, Korea; Department of Nuclear Medicine, Asan Medical Center, University of Ulsan College of Medicine, Korea; Department of Nuclear Medicine, Seoul National University Bundang Hospital, Seoul National University College of Medicine, Korea. |
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