Document Detail


Evaluating the usefulness of protein structure models for molecular replacement.
MedLine Citation:
PMID:  16204129     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
MOTIVATION: We investigate the relationship between the quality of models of protein structure and their usefulness as search models in molecular replacement, a widely used method to experimentally determine protein structures by X-ray crystallography. RESULTS: We used the available models submitted to the Critical Assessment of Techniques for Protein Structure Prediction to verify in which cases they can be automatically used as search templates for molecular replacement. Our results show that there is a correlation between the quality of the models and their suitability for molecular replacement but that the traditional method of relying on sequence identity between the model and the template used to build it is not diagnostic for the success of the procedure. AVAILABILITY: Additional data are available at http://cassandra.bio.uniroma1.it/mr-results-casp.html
Authors:
Alejandro Giorgetti; Domenico Raimondo; Adriana Erica Miele; Anna Tramontano
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Bioinformatics (Oxford, England)     Volume:  21 Suppl 2     ISSN:  1367-4811     ISO Abbreviation:  Bioinformatics     Publication Date:  2005 Sep 
Date Detail:
Created Date:  2005-10-05     Completed Date:  2007-08-27     Revised Date:  2009-11-04    
Medline Journal Info:
Nlm Unique ID:  9808944     Medline TA:  Bioinformatics     Country:  England    
Other Details:
Languages:  eng     Pagination:  ii72-6     Citation Subset:  IM    
Affiliation:
Department of Biochemical Sciences, University of Rome La Sapienza, Rome, Italy.
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MeSH Terms
Descriptor/Qualifier:
Algorithms*
Amino Acid Sequence
Amino Acid Substitution
Computer Simulation
Crystallography, X-Ray / methods*
Models, Chemical*
Models, Molecular*
Molecular Sequence Data
Protein Conformation
Proteins / chemistry*,  ultrastructure*
Sequence Analysis, Protein / methods*
Chemical
Reg. No./Substance:
0/Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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