Document Detail


Evaluating survival of Escherichia coli O157:H7 in frozen and thawed apple cider: potential use of a hydrophobic grid membrane filter-SD-39 agar method.
MedLine Citation:
PMID:  9709216     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To determine the susceptibility of Escherichia coli O157:H7 to freezing and thawing in apple cider, methods that recover injured cells are needed for accurate enumeration. This study compared the ISO-GRID hydrophobic grid membrane filter (HGMF) SD-39 agar method to two other methods: a reference most probable number (MPN) method, and plating on sorbitol MacConkey agar (SMA). To determine numbers of injured cells, SMA spread plating was also compared to Trypticase soy agar (TSA) spread plating. Two strains of E. coli O157:H7 QA 326 and ATCC 43895, were inoculated into presterilized apple cider (10 ml) which was then frozen (-20 degrees C for 24 h). Samples were thawed at 4 degrees C for 4 h, or at 23 degrees C for 1.5 h, or in a microwave oven (700 W for 10 s). Substantial cell death (0.69- to 6.33 log10 CFU/ml decreases) and injury (0.70- to 2.38-log10 CFU/ml decreases) occurred during freezing and thawing. The extent of death and injury varied with strain and thawing method. The TSA spread plating method recovered the most cells while the HGMF method always recovered more viable cells than the reference MPN method and also either recovered significantly more (P < 0.05) cells or a not significantly different number of cells than SMA spread plating. Some injured cells of both strains were not counted by the HGMF method. Significant numbers of cells injured by freezing and thawing at 4 degrees C in apple cider were enumerated in the cider was diluted 1:2 Trypticase soy broth immediately before plating. Two epifluorescent microscopic methods showed that injury was not associated with loss of cell membrane integrity.
Authors:
J R Sage; S C Ingham
Related Documents :
11791546 - An "optical channel": a technique for the evaluation of biological cell elasticity.
20407226 - Slow cooling cryopreservation of cell-microcarrier constructs.
6165216 - Sickle cell traits in canada. trimodal distribution of hb s as a result of interaction ...
17884036 - Cell partitioning during the directional solidification of trehalose solutions.
9681876 - Age-dependent changes and interrelations of number of cells and biochemical parameters ...
476806 - Chloride cells and chloride exchange in the skin of a sea-water teleost, the shanny (bl...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of food protection     Volume:  61     ISSN:  0362-028X     ISO Abbreviation:  J. Food Prot.     Publication Date:  1998 Apr 
Date Detail:
Created Date:  1998-09-17     Completed Date:  1998-09-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7703944     Medline TA:  J Food Prot     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  490-4     Citation Subset:  IM    
Affiliation:
University of Wisconsin-Madison, Department of Food Science 53706-1565, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Agar
Bacteriological Techniques
Beverages / microbiology*
Colony Count, Microbial
Culture Media
Escherichia coli O157 / growth & development*,  isolation & purification
Filtration / methods
Freezing*
Membranes, Artificial
Rosales / microbiology*
Chemical
Reg. No./Substance:
0/Culture Media; 0/Membranes, Artificial; 9002-18-0/Agar

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Chilling and trimming effects on the microbial populations of pork carcasses.
Next Document:  Role of organic acids during processing to improve quality of channel catfish fillets.