| Evaluating the localization and DNA binding complexity of histones in mature sperm. | |
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MedLine Citation:
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PMID: 22992937 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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The paternal genome in many animal taxa is efficiently packaged into the sperm nucleus by switching from a histone (nucleosome)-based chromatin configuration to one using predominantly protamines. Nonetheless, various studies have shown that some nucleosomes, often containing modified histones are retained in mature sperm and bind DNA with distinct sequence compositions. Considering the significance of histone modifications in epigenetic phenomena and the fact that sperm histones and their bound DNA must be carried into the oocyte, this chapter describes methods aimed at examining and analysing the histone composition of sperm chromatin. The focus is on both microscopic visualisation and evaluation of sequence composition of histones and histone-bound DNA in human and mouse spermatozoa. However, similar methods may be applicable to the sperm of other mammalian and even non-mammalian classes. |
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Authors:
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David Miller; Agnieszka Paradowska |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 927 ISSN: 1940-6029 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2013 |
Date Detail:
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Created Date: 2012-09-20 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 459-75 Citation Subset: IM |
Affiliation:
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Leeds Institute of Genetics, Health and Therapeutics, University of Leeds, Leeds, West Yorkshire, UK, D.Miller@leeds.ac.uk. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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