Document Detail


Evaluating the laboratory techniques used in the diagnosis of sputum-producing-patients suspected of Mycobacterium infection.
MedLine Citation:
PMID:  9643159     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Because of the susceptibility of HIV-infected persons to Mycobacterial infections, more sensitive diagnostic technique are being employed to detect Mycobacterium spp from clinical specimens. This study was therefore carried out to evaluate the sensitivity of methods used for the diagnosis of mycobacterial infections among sputum producing patients. Sputum samples from 160 patients were examined for Mycobacterium spp using direct smear microscopy, concentrated smear microscopy, and cultural method. The cultural technique detected the highest number of positives, 62 (38.8%), followed by concentrated smear technique,, 41 (25.6%) while direct smear technique detected only 24 (15.0%). The results show that cultural technique should be employed for the diagnosis of Mycobacterium in order to avoid false negative results. However, concentrated smear technique should always be carried out, so that treatment could commence immediately in obviously positive cases.
Authors:
N C Ukwandu; J O Chikwen; A E Moses
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  West African journal of medicine     Volume:  17     ISSN:  0189-160X     ISO Abbreviation:  West Afr J Med     Publication Date:    1998 Jan-Mar
Date Detail:
Created Date:  1998-07-09     Completed Date:  1998-07-09     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8301891     Medline TA:  West Afr J Med     Country:  NIGERIA    
Other Details:
Languages:  eng     Pagination:  38-41     Citation Subset:  IM; X    
Affiliation:
Department of Medical Microbiology, College of Medical Sciences, University of Maiduguri, Nigeria.
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MeSH Terms
Descriptor/Qualifier:
AIDS-Related Opportunistic Infections / diagnosis,  microbiology
Adolescent
Adult
Age Distribution
Bacteriological Techniques / standards*
Child
Cytodiagnosis / standards*
False Negative Reactions
Female
Humans
Male
Middle Aged
Mycobacterium Infections / diagnosis*,  microbiology*
Reproducibility of Results
Sensitivity and Specificity
Sex Distribution
Sputum / microbiology*
Comments/Corrections
Erratum In:
West Afr J Med 1998 Oct-Dec;17(4):291

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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