| Evaluating high time-resolved changes in carbon isotope ratio of respired CO2 by a rapid in-tube incubation technique. | |
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MedLine Citation:
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PMID: 17348086 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Recent insights into fractionation during dark respiration and rapid dynamics in isotope signatures of leaf- and ecosystem-respired CO(2) indicate the need for new methods for high time-resolved measurements of the isotopic signature of respired CO(2) (delta(13)C(res)). We present a rapid and simple method to analyse delta(13)C(res) using an in-tube incubation technique and an autosampler for small septum-capped vials. The effect of storage on the delta(18)O and delta(13)C ratios of ambient CO(2) concentrations was tested with different humidity and temperatures. delta(13)C ratios remained stable over 72 h, whereas delta(18)O ratios decreased after 24 h. Storage at 4 degrees C improved the storage time for delta(18)O. Leaves or leaf discs were incubated in the vials, flushed with CO(2)-free air and respired CO(2) was automatically sampled within 5 min on a microGas autosampler interfaced to a GV-Isoprime isotope ratio mass spectrometer. Results were validated by simultaneous on-line gas-exchange measurements of delta(13)C(res) of attached leaves. This method was used to evaluate the short-term (5-60 min) and diurnal dynamics of delta(13)C(res) in an evergreen oak (Quercus ilex) and a herb (Tolpis barbata). An immediate depletion of 2-4 per thousand from the initial delta(13)C(res) value occurred during the first 30 min of darkening. Q. ilex exhibited further a substantial diurnal enrichment in delta(13)C(res) of 8 per thousand, followed by a progressive depletion during the night. In contrast, T. barbata did not exhibit a distinct diurnal pattern. This is in accordance with recent theory on fractionation in metabolic pathways and may be related to the different utilisation of the respiratory substrate in the fast-growing herb and the evergreen oak. These data indicate substantial and rapid dynamics (within minutes to hours) in delta(13)C(res), which differed between species and probably the growth status of the plant. The in-tube incubation method enables both high time-resolved analysis and extensive sampling across different organs, species and functional types. |
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Authors:
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Christiane Werner; Nils Hasenbein; Rodrigo Maia; Wolfram Beyschlag; Cristina Máguas |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Rapid communications in mass spectrometry : RCM Volume: 21 ISSN: 0951-4198 ISO Abbreviation: Rapid Commun. Mass Spectrom. Publication Date: 2007 |
Date Detail:
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Created Date: 2007-05-07 Completed Date: 2007-05-29 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8802365 Medline TA: Rapid Commun Mass Spectrom Country: England |
Other Details:
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Languages: eng Pagination: 1352-60 Citation Subset: IM |
Copyright Information:
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Copyright (c) 2007 John Wiley & Sons, Ltd. |
Affiliation:
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Experimental and Systems Ecology, University of Bielefeld, Universitätsstrasse 25, D-33615 Bielefeld, Germany. c.werner@uni-bielefeld.de |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Carbon Dioxide
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chemistry,
metabolism* Carbon Isotopes / analysis, metabolism* Chromatography, Gas / instrumentation, methods Circadian Rhythm Darkness Ecosystem Mass Spectrometry / instrumentation*, methods Photosynthesis / physiology Plant Leaves / metabolism* Reproducibility of Results Respiration Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Carbon Isotopes; 124-38-9/Carbon Dioxide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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