Document Detail

Evaluating the UVA photoprotection of sunscreens with murine skin edema.
MedLine Citation:
PMID:  1732382     Owner:  NLM     Status:  MEDLINE    
The acute and chronic deleterious effects of UVA on skin have prompted a growing interest in developing effective UVA-photoprotective sunscreens. The quantification of their UVA photoprotection remains, however, a major problem. In the present study, murine skin edema induced by 8-methoxypsoralen plus UVA (PUVA) is evaluated as a screening method for quantifying the UVA photoprotection of commercially available sunscreens. The PUVA-induced murine skin edema is provoked on the dorsa of female hairless albino mice and measured with a hand-held micrometer. A clear time course and a well-defined dose-response relationship are demonstrated. Therefore, a UVA-photoprotection factor (UVA-PF) could be defined by dividing the minimal edema dose with sunscreen by the minimal edema dose without sunscreen. The UVA-PF values obtained with this method were quantitatively and qualitatively very similar to those obtained in 8-methoxypsoralen-photosensitized murine skin by using the number of sunburn cells as the biologic end point and were qualitatively similar to UVA-PF values obtained in human skin using phototoxic erythema and UVASUN-induced tanning as the parameter. It is concluded that PUVA-induced murine skin edema offers an objective, reproducible, and easily applicable screening method for quantifying the degree of UVA photoprotection of a sunscreen.
M Garmyn; R Roelandts
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of investigative dermatology     Volume:  98     ISSN:  0022-202X     ISO Abbreviation:  J. Invest. Dermatol.     Publication Date:  1992 Feb 
Date Detail:
Created Date:  1992-02-27     Completed Date:  1992-02-27     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0426720     Medline TA:  J Invest Dermatol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  187-90     Citation Subset:  IM    
Photodermatology Unit, University of Leuven, Belgium.
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MeSH Terms
Edema / drug therapy*,  etiology
Evaluation Studies as Topic
PUVA Therapy
Skin / radiation effects*
Skin Diseases / drug therapy*,  etiology
Skinfold Thickness
Sunscreening Agents / therapeutic use*
Ultraviolet Rays*
Reg. No./Substance:
0/Sunscreening Agents

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