Document Detail

Evaluating revascularization and flap survival using vascular endothelial growth factor in an irradiated rat model.
MedLine Citation:
PMID:  21242418     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To evaluate the role of vascular endothelial growth factor (VEGF) plasmid DNA (pDNA) in improving flap revascularization in a previously developed rat model. Our hypothesis was that the uptake and expression of VEGF pDNA in the wound bed would improve revascularization and flap viability.
DESIGN: Twenty-eight male Sprague-Dawley rats received a total dose of 40 Gy electron beam radiation to the ventral abdominal wall. After a recovery period, they underwent a ventral fasciocutaneous flap procedure with a 2-hour ischemia period. Group 1 (n = 14) received topical VEGF pDNA, in vivo cationic polymer, and fibrin sealant. Group 2 (n = 14) received topical cationic polymer and fibrin sealant only. Seven of the rats from each group underwent pedicle ligation at 8 or 14 days. The primary outcome measure was percentage of flap revascularization 5 days after pedicle ligation.
RESULTS: Rats receiving VEGF pDNA had a significantly higher rate of flap revascularization (90.8% vs 79.8%) after pedicle ligation at 14 days (P = .045). At 8 days, rats receiving VEGF pDNA (group 1) had an increased rate of flap revascularization (58.2% vs 42.8%) that approached significance (P = .11).
CONCLUSION: This study demonstrates the potential of VEGF pDNA to improve revascularization and flap viability in previously irradiated tissue.
Patrick C Angelos; Shelley R Winn; Darryl S Kaurin; John Holland; Mark K Wax
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Publication Detail:
Type:  Journal Article     Date:  2011-01-17
Journal Detail:
Title:  Archives of facial plastic surgery     Volume:  13     ISSN:  1538-3660     ISO Abbreviation:  Arch Facial Plast Surg     Publication Date:    2011 May-Jun
Date Detail:
Created Date:  2011-05-17     Completed Date:  2011-10-13     Revised Date:  2012-09-24    
Medline Journal Info:
Nlm Unique ID:  100883500     Medline TA:  Arch Facial Plast Surg     Country:  United States    
Other Details:
Languages:  eng     Pagination:  185-9     Citation Subset:  IM    
Department of Otolaryngology-Head and Neck Surgery, Oregon Health & Science University, Mail Code PV01, 3181 SW Sam Jackson Park Rd, Portland, OR 29239, USA.
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MeSH Terms
Administration, Cutaneous
Free Tissue Flaps / blood supply*
Gene Expression
Gene Transfer Techniques*
Graft Survival / drug effects*,  radiation effects
Models, Animal
Rats, Sprague-Dawley
Vascular Endothelial Growth Factors / genetics*,  pharmacology
Reg. No./Substance:
0/Vascular Endothelial Growth Factors

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