Document Detail


Evaluating DSAEK graft deturgescence in preservation medium after microkeratome cut with optical coherence tomography.
MedLine Citation:
PMID:  23538636     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: To evaluate the Descemet stripping automated endothelial keratoplasty (DSAEK) graft deturgescence in preservation medium after microkeratome cut using Fourier domain optical coherence tomography.
METHODS: The central and peripheral thickness of DSAEK grafts was measured by Fourier domain optical coherence tomography immediately after microkeratome cuts and 1, 2, 3, and 4 hours afterward. All measurements were taken when the grafts were stored in 4°C preservation medium. The hourly change in central graft thickness and graft shape (peripheral graft thicknes - central graft thickness) was calculated and tracked over time.
RESULTS: Five DSAEK grafts were measured. The average central graft thickness was 188.7 ± 44.4 μm (range, 146-255 µm) immediately after microkeratome cuts. The average central graft thickness was 147.5 ± 33.0 μm (range, 116-190 µm) after 4 hours in preservation medium (P < 0.001). The average hourly change in central graft thickness was -30.5 μm (P = 0.0051), -8.6 μm (P = 0.055), -2.0 μm (P = 0.42), and 0.0 μm (P = 0.93) at 1, 2, 3, and 4 hours, respectively, after microkeratome cuts. The average hourly change in graft shape was insignificant.
CONCLUSIONS: DSAEK grafts become thinner after microkeratome cut and stabilize at approximately 2 hours. Therefore, DSAEK graft thickness should be measured at 1.5 to 3 hours after microkeratome cut.
Authors:
Maolong Tang; Christopher Stoeger; Joshua Galloway; Jeffrey Holiman; Matthew R Bald; David Huang
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cornea     Volume:  32     ISSN:  1536-4798     ISO Abbreviation:  Cornea     Publication Date:  2013 Jun 
Date Detail:
Created Date:  2013-05-10     Completed Date:  2013-09-19     Revised Date:  2014-06-03    
Medline Journal Info:
Nlm Unique ID:  8216186     Medline TA:  Cornea     Country:  United States    
Other Details:
Languages:  eng     Pagination:  847-50     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Cornea / pathology*
Cryopreservation*
Descemet Stripping Endothelial Keratoplasty*
Dilatation, Pathologic
Fourier Analysis
Humans
Organ Preservation*
Organ Preservation Solutions*
Time Factors
Tomography, Optical Coherence*
Grant Support
ID/Acronym/Agency:
R01 EY018184/EY/NEI NIH HHS; R01 EY018184/EY/NEI NIH HHS
Chemical
Reg. No./Substance:
0/Organ Preservation Solutions
Comments/Corrections

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