| European elk papillomavirus: characterization of the genome, induction of tumors in animals, and transformation in vitro. | |
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MedLine Citation:
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PMID: 6312094 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The European elk papillomavirus (EEPV) genome was cloned in the BamHI cleavage site of the pBR322 vector. The cloned genome was used for construction of a physical map, employing restriction endonucleases BamHI, BglII, HindIII, PvuII, SacI, and XhoI. The sequence homology between the EEPV and bovine papillomavirus type 1 genomes was elucidated by performing hybridizations in different concentrations of formamide. Sequence homology could only be revealed under less stringent conditions, i.e., Tm - 43 degrees C. Nucleotide sequence information was also collected from the regions which lie adjacent to the three HindIII sites that are present in the EEPV genome. The results made it possible to align the EEPV and bovine papillomavirus type 1 genomes. Transformation by EEPV was demonstrated with the C127 mouse cell line, and fibrosarcomas were induced in young hamsters after subcutaneous injection. The transformed cells and the tumors contain multiple, nonintegrated copies of the EEPV genome. Virus particles could not be detected either in tumors or in transformed cells. |
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Authors:
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A Stenlund; J Moreno-Lopez; H Ahola; U Pettersson |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of virology Volume: 48 ISSN: 0022-538X ISO Abbreviation: J. Virol. Publication Date: 1983 Nov |
Date Detail:
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Created Date: 1983-11-23 Completed Date: 1983-11-23 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0113724 Medline TA: J Virol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 370-6 Citation Subset: IM |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/K01661; K01662 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Base Sequence Bovine papillomavirus 1 / genetics Cell Line Cell Transformation, Neoplastic* Cell Transformation, Viral* Cloning, Molecular Cricetinae DNA Restriction Enzymes Deer / microbiology Fibrosarcoma / microbiology* Genes, Viral* Mesocricetus Mice Papillomaviridae / genetics, physiology* |
| Chemical | |
Reg. No./Substance:
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EC 3.1.21.-/DNA Restriction Enzymes |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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