Document Detail

European elk papillomavirus: characterization of the genome, induction of tumors in animals, and transformation in vitro.
MedLine Citation:
PMID:  6312094     Owner:  NLM     Status:  MEDLINE    
The European elk papillomavirus (EEPV) genome was cloned in the BamHI cleavage site of the pBR322 vector. The cloned genome was used for construction of a physical map, employing restriction endonucleases BamHI, BglII, HindIII, PvuII, SacI, and XhoI. The sequence homology between the EEPV and bovine papillomavirus type 1 genomes was elucidated by performing hybridizations in different concentrations of formamide. Sequence homology could only be revealed under less stringent conditions, i.e., Tm - 43 degrees C. Nucleotide sequence information was also collected from the regions which lie adjacent to the three HindIII sites that are present in the EEPV genome. The results made it possible to align the EEPV and bovine papillomavirus type 1 genomes. Transformation by EEPV was demonstrated with the C127 mouse cell line, and fibrosarcomas were induced in young hamsters after subcutaneous injection. The transformed cells and the tumors contain multiple, nonintegrated copies of the EEPV genome. Virus particles could not be detected either in tumors or in transformed cells.
A Stenlund; J Moreno-Lopez; H Ahola; U Pettersson
Related Documents :
21252984 - Targeted next-generation sequencing detects point mutations, insertions, deletions and ...
7890174 - Complete sequence of the ovine beta-casein-encoding gene and interspecies comparison.
21304634 - Complete genome sequence of desulfomicrobium baculatum type strain (x).
24522484 - A pd-catalyzed one-pot dehydrogenative aromatization and ortho-functionalization sequen...
6193114 - Nucleotide sequence of dictyostelium small nuclear rna dd8 not homologous to any other ...
8672814 - Cloning and characterization of a melanin biosynthetic thr1 reductase gene essential fo...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of virology     Volume:  48     ISSN:  0022-538X     ISO Abbreviation:  J. Virol.     Publication Date:  1983 Nov 
Date Detail:
Created Date:  1983-11-23     Completed Date:  1983-11-23     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0113724     Medline TA:  J Virol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  370-6     Citation Subset:  IM    
Data Bank Information
Bank Name/Acc. No.:
GENBANK/K01661;  K01662
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Base Sequence
Bovine papillomavirus 1 / genetics
Cell Line
Cell Transformation, Neoplastic*
Cell Transformation, Viral*
Cloning, Molecular
DNA Restriction Enzymes
Deer / microbiology
Fibrosarcoma / microbiology*
Genes, Viral*
Papillomaviridae / genetics,  physiology*
Reg. No./Substance:
EC 3.1.21.-/DNA Restriction Enzymes

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Characteristics and regulation of interaction of avian retrovirus pp12 protein with viral RNA.
Next Document:  Virus-specific transcription in a Herpesvirus saimiri-transformed lymphoid tumor cell line.