| Euro-Collins solution versus UW-solution for long-term liver preservation in the isolated rat-liver perfusion model. | |
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MedLine Citation:
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PMID: 1810373 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To compare UW-solution (UW) and Euro-Collins (EC) for long-term liver preservation we investigated the morphology and metabolic capacity of rat liver after 18 and 42-hours cold-storage in either UW or EC. After harvesting the rat liver was transferred to a perfusion chamber where it was perfused for 10 min with UW or EC at 4 degrees C. Thereafter livers were stored at 4 degrees C in UW or EC for 18 hours (both groups n = 6) or for 42 hours (both groups n = 8). After 18-hr or 42-hr cold-storage a 2-hr warm perfusion (37 degrees C) was started with Krebs-Ringer solution with carbogen to which 125Iodine-triiodothyronine (T3) was added. Control livers (n = 8) were immediately perfused with Krebs-Ringer without cold-storage. The following parameters were assessed: ASAT-levels in the perfusate, T3-metabolites in the bile and the perfusate, the perfusion pressure, the volume of bile secreted and light-microscopical morphology at the end of the warm perfusion period. After cold storage in UW-solution the ASAT-levels in the perfusate were lower than after storage in EC as well as the perfusion pressures. These livers demonstrated a better T3-metabolism and secreted more bile than EC-stored livers. Histological examination showed more tissue damage in the EC-stored livers than in the UW stored livers. We conclude that cold-storage of rat liver in UW-solution resulted in a better morphology and metabolic capacity as compared with EC-solution. |
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Authors:
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R Den Toom; M De Jong; E P Krenning; H J Van der Hoek; F J Ten Kate; G Hennemann; O T Terpstra |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: HPB surgery : a world journal of hepatic, pancreatic and biliary surgery Volume: 4 ISSN: 0894-8569 ISO Abbreviation: HPB Surg Publication Date: 1991 |
Date Detail:
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Created Date: 1992-06-03 Completed Date: 1992-06-03 Revised Date: 2008-11-20 |
Medline Journal Info:
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Nlm Unique ID: 9002972 Medline TA: HPB Surg Country: SWITZERLAND |
Other Details:
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Languages: eng Pagination: 313-20 Citation Subset: IM |
Affiliation:
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Department of Surgery, University Hospital Dijkzigt, Erasmus University Rotterdam, The Netherlands. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Allopurinol Animals Aspartate Aminotransferases / metabolism Bile / secretion Drug Evaluation, Preclinical Glutathione Hypertonic Solutions / standards* Insulin Liver / anatomy & histology*, metabolism Male Organ Preservation Solutions* Raffinose Rats Rats, Inbred Strains Reperfusion* Solutions / standards* Tissue Preservation / methods, standards* Triiodothyronine / metabolism, pharmacokinetics |
| Chemical | |
Reg. No./Substance:
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0/Euro-Collins' solution; 0/Hypertonic Solutions; 0/Organ Preservation Solutions; 0/Solutions; 0/University of Wisconsin-lactobionate solution; 11061-68-0/Insulin; 315-30-0/Allopurinol; 512-69-6/Raffinose; 58-61-7/Adenosine; 6893-02-3/Triiodothyronine; 70-18-8/Glutathione; EC 2.6.1.1/Aspartate Aminotransferases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
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Journal Information Journal ID (nlm-ta): HPB Surg ISSN: 0894-8569 ISSN: 1607-8462 Publisher: Hindawi Publishing Corporation |
Article Information Download PDF  Copyright ? 1991 Hindawi Publishing Corporation. open-access: This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Received Day: 15 Month: 3 Year: 1991 Accepted Day: 23 Month: 4 Year: 1991 Print publication date: Year: 1991 Volume: 4 Issue: 4 First Page: 313 Last Page: 320 ID: 2423642 PubMed Id: 1810373 Publisher Item Identifier: S0894856991109659 DOI: 10.1155/1991/10965 |
| Euro-Collins Solution Versus Uw-Solution for Long-Term Liver Preservation in the Isolated Rat-Liver Perfusion Model | |
| Ren? Den Toom1 | |
| Marion De Jong2 | |
| Eric P. Krenning2 | |
| Hans J. Van Der Hoek2 | |
| Fiebo J. W. Ten Kate3 | |
| Georg Hennemann2 | |
| Onno T. Terpstra1 | |
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1Departments of SurgeryUniversity Hospital?Dijkzigt?Erasmus University RotterdamDr Molewaterplein 40Rotterdam3015 GDThe Netherlands |
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2Departments of Internal Medicine IIIUniversity Hospital?Dijkzigt?Erasmus University RotterdamRotterdamThe Netherlands |
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3Departments of PathologyUniversity Hospital?Dijkzigt?Erasmus University RotterdamRotterdamThe Netherlands |
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