| Eupafolin, a flavonoid isolated from Artemisia princeps, induced apoptosis in human cervical adenocarcinoma HeLa cells. | |
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MedLine Citation:
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PMID: 20397191 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Although eupafolin, a flavone found in Artemisia princeps Pampanini, has been shown to inhibit the growth of several human cancer cells, its mode of action is poorly understood. In this study, we investigated the pro-apoptotic activities of eupafolin in human cervical carcinoma HeLa cells. It was found that eupafolin induced apoptosis in a dose-dependent manner, as evidenced by DNA fragmentation and the accumulation of positive cells for annexin V. In addition, eupafolin triggered the activations of caspases-3, -6, -7, -8, and -9 and the cleavages of their substrates, such as, poly (ADP-ribose) polymerase and lamin A/C. Furthermore, treatment with eupafolin resulted in a loss of mitochondrial membrane potential (DeltaPsi(m)), increased the release of cytochrome c to the cytosol, and altered the expression levels of B-cell lymphoma 2 (Bcl-2) family proteins. Interestingly, caspase-8, an initiator caspase, was activated after the loss of DeltaPsi(m) and the activations of caspases-3 and -9. Moreover, treatment with z-DEVD-fmk (a specific caspase-3 inhibitor) and the overexpression of Bcl-2 prevented eupafolin-stimulated caspase-8 activation. Altogether, these results suggest that the eupafolin-induced apoptosis in HeLa cells is mediated by caspase-dependent pathways, involving caspases-3, -9, and -8, which are initiated by the Bcl-2-dependent loss of DeltaPsi(m). |
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Authors:
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Kyung-Sook Chung; Jung-Hye Choi; Nam-In Back; Myung-Sook Choi; Eun-Kyung Kang; Hae-Gon Chung; Tae-Sook Jeong; Kyung-Tae Lee |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Molecular nutrition & food research Volume: 54 ISSN: 1613-4133 ISO Abbreviation: Mol Nutr Food Res Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-09-08 Completed Date: 2011-01-27 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101231818 Medline TA: Mol Nutr Food Res Country: Germany |
Other Details:
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Languages: eng Pagination: 1318-28 Citation Subset: IM |
Affiliation:
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Department of Biomedical Science, College of Medical Science, Kyung-Hee University, Seoul, South Korea. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenocarcinoma
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drug therapy* Antineoplastic Agents, Phytogenic / chemistry, isolation & purification, pharmacology* Apoptosis / drug effects* Artemisia / chemistry* Caspases / metabolism Cell Line, Tumor DNA Fragmentation / drug effects Down-Regulation / drug effects Enzyme Activation / drug effects Female Flavones / chemistry, isolation & purification, pharmacology* Hela Cells Humans Inhibitory Concentration 50 Kinetics Membrane Potential, Mitochondrial / drug effects Mitochondria / drug effects, enzymology Permeability / drug effects Proto-Oncogene Proteins c-bcl-2 / genetics, metabolism Uterine Cervical Neoplasms / drug therapy* |
| Chemical | |
Reg. No./Substance:
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0/Antineoplastic Agents, Phytogenic; 0/Flavones; 0/Proto-Oncogene Proteins c-bcl-2; 0/eupafolin; EC 3.4.22.-/Caspases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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