| Euchresta horsfieldii Benn. activates peroxisome proliferator-activated receptor α and regulates expression of genes involved in fatty acid metabolism in human HepG2 cells. | |
| | |
MedLine Citation:
|
PMID: 20920566 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
|
AIM OF THE STUDY: Euchresta horsfieldii Benn., an oriental medicinal plant, has been used for the traditional treatment of hyperlipidemia and has been reported to possess bioactive isoflavones; however, the molecular mechanism underlying its hypolipidemic effects remains unclear. In the present study, we investigated the effect of Euchresta horsfieldii on peroxisome proliferator-activated receptor α (PPARα) activation and fatty acid metabolism in HepG2 hepatocytes. MATERIALS AND METHODS: The dried Euchresta horsfieldii fruits were extracted with 100% ethanol, and the ethanol evaporated to produce Euchresta horsfieldii extract (EHX). The effect of EHX on fatty acid metabolism was evaluated by PPARα transactivation assay, real-time reverse transcription-polymerase chain reaction, and Western blot analysis. RESULTS: We demonstrated that EHX significantly increased PPARα activation in a dose-dependent manner. In human HepG2 hepatocytes, EHX increased mRNA levels of the following genes involved in fatty acid oxidation: carnitine palmitoyltransferase 1, liver form (CPT1L), acyl-CoA synthetase (ACS), medium-chain acyl-CoA dehydrogenase (MCAD), 3-hydroxy-3-methylglutaryl-CoA synthase 2 (HMGCS2), acyl-CoA 1 (ACO1), acyl-CoA 2 (ACO2), and enoyl-CoA hydratase 1 (ECH1). EHX treatment also increased levels of proteins related to fatty acid oxidation, such as CPT1L, PPARα, and uncoupling protein 2 (UCP2). In contrast, sterol regulatory element binding protein 1 (SREBP1), a key lipogenic transcription factor, was downregulated. CONCLUSION: Consistent with significant PPARα activation, EHX increased PPARα target genes expression and regulated protein expression for lipid metabolism. Taken together, these results indicate that Euchresta horsfieldii shows potential as a natural lipid-lowering agent. |
| | |
Authors:
|
Jeong-Hwan Kim; Daeyoung Kim; Jaekyung Kim; Jae-Kwan Hwang |
Related Documents
:
|
861236 - Unsaturated fatty acyl-coa inhibition of cholesterol synthesis in vitro. 8218206 - Site-directed mutagenesis of putative active-site amino acid residues of 3,2-trans-enoy... 9250376 - Decompensation of hepatic and cerebral acyl-coa metabolism in balb/cbyj mice by chronic... 8399326 - Purification of peroxisomes and subcellular distribution of enzyme activities for activ... 11375016 - Lewis acid-promoted deoxygenative di[beta,beta-bis(ethylthio)]vinylation of aldehydes w... 12162346 - The ontogeny of fatty acid-binding protein in turkey (meleagridis gallopavo) intestine ... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. Date: 2010-10-20 |
Journal Detail:
|
Title: Journal of ethnopharmacology Volume: 133 ISSN: 1872-7573 ISO Abbreviation: J Ethnopharmacol Publication Date: 2011 Jan |
Date Detail:
|
Created Date: 2010-12-27 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 7903310 Medline TA: J Ethnopharmacol Country: Ireland |
Other Details:
|
Languages: eng Pagination: 244-7 Citation Subset: IM |
Copyright Information:
|
Copyright © 2010 Elsevier Ireland Ltd. All rights reserved. |
Affiliation:
|
Department of Biotechnology, Yonsei University, Seoul, Republic of Korea. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Anti-angiogenesis effect of essential oil from Curcuma zedoaria in vitro and in vivo.
Next Document: Pentacyclic triterpenoids and ceramide mediate the vasorelaxant activity of Vitex cienkowskii via in...