Document Detail


Eubacteria have 3 growth modes keyed to nutrient flow. Consequences for the concept of maintenance and maximal growth yield.
MedLine Citation:
PMID:  6202266     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Aerobic growth of Escherichia coli and Paracoccus denitrificans has been studied in chemostat, fed batch, and recycling fermentor modes under carbon and energy limitation. Two abrupt drops or discontinuities in molar growth yield, Y, have been found that occur over relatively short ranges in the value of specific growth rate. Before the first discontinuity, Y is constant and maximal. After the first discontinuity, at a doubling time of 33 h, Y becomes constant again and independent of mu until the second discontinuity appears at a doubling time of about 50 h, corresponding to a mu of about 0.014. At this point, Y drops to a lower value that is constant at doubling times longer than 100 h, corresponding to a mu of about 0.007. The second discontinuity is associated in Paracoccus with elevated levels of guanosine tetraphosphate (ppGpp) that impose stringent regulation as has been found previously with Bacillus and Escherichia species. It is thus likely that the stringent response generally occurs in bacteria in vivo at a doubling time of about 50 h. The cause of the first discontinuity is unknown. All experiments indicate that Pirt-type calculations relating mu, Y, and maintenance energy demand are no longer valid. In chemostat experiments, the intercept of the relationship between specific substrate utilization and specific growth rate is defined as maintenance. However, this intercept most probably is caused by stringent regulation at low dilution rates. Three regions of bacterial growth rates are defined by this study, corresponding to doubling times of 0.5 to 15 h, 33 to 50 h, and greater than 100 h. Some growth behavior in each region is unique to that region.
Authors:
H W van Verseveld; W R Chesbro; M Braster; A H Stouthamer
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Archives of microbiology     Volume:  137     ISSN:  0302-8933     ISO Abbreviation:  Arch. Microbiol.     Publication Date:  1984 Feb 
Date Detail:
Created Date:  1984-06-18     Completed Date:  1984-06-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0410427     Medline TA:  Arch Microbiol     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  176-84     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Chromatography, Thin Layer
Ecology
Escherichia coli / growth & development*,  metabolism
Fermentation
Guanosine Tetraphosphate / metabolism
Mathematics
Oxygen Consumption
Paracoccus denitrificans / growth & development*,  metabolism
RNA, Bacterial / biosynthesis
Chemical
Reg. No./Substance:
0/RNA, Bacterial; 33503-72-9/Guanosine Tetraphosphate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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