Document Detail


Ethylglucuronide and ethylsulfate in meconium to assess gestational ethanol exposure: preliminary results in two Mediterranean cohorts.
MedLine Citation:
PMID:  19553703     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: In recent years, fatty acid ethyl esters (FAEEs) in meconium emerged as reliable, direct biological markers for establishing gestational ethanol exposure. Among the minor nonoxidative products of ethanol metabolism, there are ethyl glucuronide (EtG) and ethyl sulfate (EtS). OBJECTIVES: The aim of the study was to analyse meconium specimens from two different Mediterranean cohorts to check for the presence of EtG and EtS, and to investigate the eventual correlation between meconium FAEEs and these two metabolites and their possible application as direct biomarkers of gestational ethanol exposure. METHODS: FAEEs, EtG and EtS were quantified by liquid chromatography tandem mass spectrometry in meconium samples obtained from the Neonatal Intensive Care Unit of Arcispedale Santa Maria Nuova, Reggio Emilia, Italy (N= 96) and from the Pediatric Service of the Hospital del Mar in Barcelona, Spain (N=81). RESULTS: EtG was present in more than 80% meconium samples while EtS only in 50% specimens Although the samples from Spain and Italy originated from similar socio-demographic cohort, EtG values in the Barcelona samples (median value: 101.5 ng/g) were statistically higher than those from Reggio Emilia ones (median value: 15.6 ng/g). In the Barcelona cohort, EtG values could differentiate between samples with FAEEs below and those equal or above 2 nmol/g - the cut-off used to differentiate heavy maternal ethanol consumption during pregnancy from occasional or no use. CONCLUSION: For the first time the presence of EtG and EtS in meconium has been proven, with EtG concentration likely to discriminate heavy maternal ethanol consumption during pregnancy disclosed by FAEEs concentration in this matrix. Further investigations are needed to verify the use of these two ethanol metabolites as alternative biomarkers of chronic in utero exposure to ethanol.
Authors:
Simona Pichini; Luca Morini; Emilia Marchei; Ilaria Palmi; Maria Concetta Rotolo; Federica Vagnarelli; Oscar Garcia-Algar; Oriol Vall; Piergiorgio Zuccaro
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Publication Detail:
Type:  Journal Article     Date:  2009-06-24
Journal Detail:
Title:  The Canadian journal of clinical pharmacology = Journal canadien de pharmacologie clinique     Volume:  16     ISSN:  1710-6222     ISO Abbreviation:  Can J Clin Pharmacol     Publication Date:  2009  
Date Detail:
Created Date:  2009-06-25     Completed Date:  2009-09-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9804162     Medline TA:  Can J Clin Pharmacol     Country:  Canada    
Other Details:
Languages:  eng     Pagination:  e370-5     Citation Subset:  IM    
Affiliation:
1Department of Therapeutic Research and Medicine Evaluation, Istituto Superiore di Sanitá, V.le Regina Elena 299, 00161 Roma, Italy. simona.pichini@iss.it
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MeSH Terms
Descriptor/Qualifier:
Adult
Alcohol Drinking / metabolism
Biological Markers
Chromatography, Liquid / methods
Cohort Studies
Ethanol / metabolism*
Female
Glucuronates / analysis*
Humans
Infant, Newborn
Italy
Male
Meconium / chemistry*
Mediterranean Region
Pregnancy
Spain
Substance Abuse Detection / methods
Sulfuric Acid Esters / analysis*
Tandem Mass Spectrometry / methods
Young Adult
Chemical
Reg. No./Substance:
0/Biological Markers; 0/Glucuronates; 0/Sulfuric Acid Esters; 17685-04-0/ethyl glucuronide; 64-17-5/Ethanol; 64-67-5/diethyl sulfate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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