Document Detail


Ethylene-responsive element binding protein (EREBP) expression and the transcriptional regulation of class I beta-1,3-glucanase during tobacco seed germination.
MedLine Citation:
PMID:  9862496     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Class I beta-1,3-glucanase (betaGLU I) is transcriptionally induced in the micropylar endosperm just before its rupture prior to the germination (i.e. radicle emergence) of Nicotiana tabacum L. cv. 'Havana 425' seeds. Ethylene is involved in endosperm rupture and high-level betaGLU I expression; but, it does not affect the spatial and temporal pattern of betaGLU I expression. A promoter deletion analysis of the tobacco betaGLU I B gene suggests that (1) the distal - 1452 to - 1193 region, which contains the positively acting ethylene-responsive element (ERE), is required for high-level, ethylene-sensitive expression, (2) the regions - 1452 to - 1193 and -402 to 0 contribute to downregulation by abscisic acid (ABA), and (3) the region -402 to -211 is necessary and sufficient for low-level micropylar-endosperm-specific expression. Transcripts of the ERE-binding proteins (EREBPs) showed a novel pattern of expression during seed germination: light or gibberellin was required for EREBP-3 and EREBP-4 expression; EREBP-4 expression was constitutive and unaffected by ABA or ethylene; EREBP-3 showed transient induction just before endosperm rupture, which was earlier in ethylene-treated seeds and inhibited by ABA. No expression of EREBP- and EREBP-2 was detected. In contrast to betaGLU I, EREBP-3 and EREBP-4 were not expressed specifically in the micropylar endosperm. The results suggest that transcriptional regulation of betaGLU I could depend on: activation of ethylene signalling pathways acting via EREBP-3 with the ERE as the target, and ethylene-independent signalling pathways with targets in the proximal promoter region that are likely to determine spatial and temporal patterns of expression.
Authors:
G Leubner-Metzger; L Petruzzelli; R Waldvogel; R Vögeli-Lange; F Meins
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Plant molecular biology     Volume:  38     ISSN:  0167-4412     ISO Abbreviation:  Plant Mol. Biol.     Publication Date:  1998 Nov 
Date Detail:
Created Date:  1998-12-31     Completed Date:  1998-12-31     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9106343     Medline TA:  Plant Mol Biol     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  785-95     Citation Subset:  IM    
Affiliation:
Friedrich Miescher Institute, Basel, Switzerland.
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MeSH Terms
Descriptor/Qualifier:
Abscisic Acid / pharmacology
DNA-Binding Proteins / drug effects,  genetics*
Ethylenes / pharmacology
Gene Expression Regulation, Developmental
Gene Expression Regulation, Plant
Germination / drug effects,  genetics
Gibberellins / pharmacology
Glucan 1,3-beta-Glucosidase
Plant Proteins*
Plants, Genetically Modified
Plants, Toxic*
Promoter Regions, Genetic
RNA, Messenger / drug effects,  metabolism
Seeds / drug effects,  genetics,  growth & development
Tobacco / drug effects,  genetics*,  growth & development
Transcription, Genetic
beta-Glucosidase / drug effects,  genetics*
Chemical
Reg. No./Substance:
0/DNA-Binding Proteins; 0/Ethylenes; 0/Gibberellins; 0/Plant Proteins; 0/RNA, Messenger; 164326-51-6/ethylene-responsive element binding protein; 21293-29-8/Abscisic Acid; 74-85-1/ethylene; EC 3.2.1.21/beta-Glucosidase; EC 3.2.1.58/Glucan 1,3-beta-Glucosidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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