Document Detail


Ethylene metabolism in Scots pine (Pinus sylvestris) shoots during the year.
MedLine Citation:
PMID:  11772556     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Ethylene evolution, concentrations of 1-aminocyclopropane-1-carboxylic acid (ACC) and ACC conjugates, activities of ACC synthase and ACC oxidase, and cambial growth as measured by tracheid production were monitored from November to July in 1-year-old shoots, and between July and September in current-year shoots, of Scots pine (Pinus sylvestris L.). Needles, buds and four stem parts (cortex, phloem, cambial region and mature xylem) were surveyed. Ethylene evolution was quantified by gas chromatography. Free ACC and bound ACC (after acidic hydrolysis of ACC conjugates) were quantified by combined gas chromatography-mass spectrometry, with [2H]ACC as an internal standard. Activities of ACC synthase and ACC oxidase were measured in crude protein extracts. We detected high activities of ACC synthase in needles and buds, but not in stem fractions except the phloem. In July, during the period of intensive shoot growth, we found ACC only in buds and needles. In contrast, ACC oxidase activity was high in stem tissues, particularly in the cambial region during the period of rapid tracheid production, but no ACC oxidase activity was detected in needles and buds. Nevertheless, needles evolved large amounts of ethylene. Ethylene was produced by all stem fractions, and the peak rate of ethylene evolution in the cambial region coincided with the period of maximal tracheid production. Conjugated ACC was present in every fraction except mature xylem. The concentration of conjugated ACC decreased when rates of tracheid production and ethylene evolution were high, suggesting that conjugated ACC may serve as a source for ACC in the cambial region. The regulation of ethylene biosynthesis in Scots pine shoots is discussed.
Authors:
Alla Klintborg; Leif Eklund; C H Anthony Little
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Tree physiology     Volume:  22     ISSN:  0829-318X     ISO Abbreviation:  Tree Physiol.     Publication Date:  2002 Jan 
Date Detail:
Created Date:  2002-01-04     Completed Date:  2002-09-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  100955338     Medline TA:  Tree Physiol     Country:  Canada    
Other Details:
Languages:  eng     Pagination:  59-66     Citation Subset:  IM    
Affiliation:
School of Biosciences and Process Technology, Växjö University, S-351 95 Växjö, Sweden.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Oxidoreductases / metabolism
Amino Acids, Cyclic / analysis
Ethylenes / biosynthesis,  metabolism*
Lyases / metabolism
Pinus / chemistry,  metabolism,  physiology*
Pinus sylvestris
Plant Leaves / chemistry
Plant Shoots / chemistry,  enzymology,  metabolism*
Plant Stems / chemistry
Seasons
Chemical
Reg. No./Substance:
0/Amino Acids, Cyclic; 0/Ethylenes; 22059-21-8/1-aminocyclopropane-1-carboxylic acid; 74-85-1/ethylene; EC 1.4.-/Amino Acid Oxidoreductases; EC 1.4.3.-/1-aminocyclopropane-1-carboxylic acid oxidase; EC 4.-/Lyases; EC 4.4.1.14/1-aminocyclopropanecarboxylate synthase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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