Document Detail


Ethylene activates a plasma membrane Ca(2+)-permeable channel in tobacco suspension cells.
MedLine Citation:
PMID:  17447907     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Here, the effects of the ethylene-releasing compound, ethephon, and the ethylene precursor, 1-aminocyclopropane-1-carboxylic acid (ACC), on ionic currents across plasma membranes and on the cytosolic Ca(2+) activity ([Ca(2+)](c)) of tobacco (Nicotiana tabacum) suspension cells were characterized using a patch-clamp technique and confocal laser scanning microscopy. Exposure of tobacco protoplasts to ethephon and ACC led to activation of a plasma membrane cation channel that was permeable to Ba(2+), Mg(2+) and Ca(2+), and inhibited by La(3+), Gd(3+) and Al(3+). The ethephon- and ACC-induced Ca(2+)-permeable channel was abolished by the antagonist of ethylene perception (1-metycyclopropene) and by the inhibitor of ACC synthase (aminovinylglycin), indicating that activation of the Ca(2+)-permeable channels results from ethylene. Ethephon elicited an increase in the [Ca(2+)](c) of tobacco suspension cells, as visualized by the Ca(2+)-sensitive probe Fluo-3 and confocal microscopy. The ethephon-induced elevation of [Ca(2+)](c) was markedly inhibited by Gd(3+) and BAPTA, suggesting that an influx of Ca(2+) underlies the elevation of [Ca(2+)](c). These results indicate that an elevation of [Ca(2+)](c), resulting from activation of the plasma membrane Ca(2+)-permeable channels by ethylene, is an essential component in ethylene signaling in plants.
Authors:
Min-Gui Zhao; Qiu-Ying Tian; Wen-Hao Zhang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The New phytologist     Volume:  174     ISSN:  0028-646X     ISO Abbreviation:  New Phytol.     Publication Date:  2007  
Date Detail:
Created Date:  2007-04-23     Completed Date:  2007-10-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9882884     Medline TA:  New Phytol     Country:  England    
Other Details:
Languages:  eng     Pagination:  507-15     Citation Subset:  IM    
Affiliation:
Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, the Chinese Academy of Sciences, Beijing 100093, China.
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MeSH Terms
Descriptor/Qualifier:
Calcium Channel Agonists / metabolism
Calcium Channels / metabolism*
Cell Membrane / metabolism*
Cells, Cultured
Cytosol / metabolism
Ethylenes / metabolism*
Organophosphorus Compounds / metabolism
Patch-Clamp Techniques
Plant Growth Regulators / metabolism*
Tobacco / chemistry,  cytology,  metabolism*
Chemical
Reg. No./Substance:
0/Calcium Channel Agonists; 0/Calcium Channels; 0/Ethylenes; 0/Organophosphorus Compounds; 0/Plant Growth Regulators; 16672-87-0/ethephon; 74-85-1/ethylene
Comments/Corrections
Comment In:
New Phytol. 2007;174(3):470-3   [PMID:  17447901 ]

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