| Ethyl pyruvate ameliorates albuminuria and glomerular injury in the animal model of diabetic nephropathy. | |
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MedLine Citation:
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PMID: 22129969 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Pyruvate is an endogenous anti-oxidant and anti-inflammatory substance. The present study was implemented to investigate the protective effect of ethyl pyruvate (EP) against the development and progression of diabetic nephropathy in an in-vivo and in-vitro model. Diabetic rats were prepared by injecting streptozotocin (STZ, 65mg/kg). Those that developed diabetes after 72 h were treated with ethyl pyruvate (40mg/kg) intraperitoneally. Diabetic rats without pyruvate treatment and nondiabetic rats were used for control. As an in vitro experiment, rat mesangial cells cultured primarily from Sprague-Dawley rat were treated in high glucose (HG, 50mM) or normal glucose (NG, 5 mM) conditions, and with or without pyruvate. Pyruvate-treated diabetic rats exhibited decreased albuminuria, and attenuated NADPH-dependent ROS generation. Immunohistochemistry showed reduced laminin, type IV collagen and fibronectin deposition in the glomeruli compared with nontreated diabetic rat. Parallel changes were shown in the tissue mRNA and protein expression level of MCP-1, TGF-β1, laminin, fibronectin and type IV collagen in the kidney. Concordantly, protective effects were also exhibited in the in-vitro mesangial cell culture system. These findings suggest that pyruvate protects against kidney injury via NADPH oxidase inhibition. The present study established that activation of the NADPH oxidase plays a crucial role in diabetes-induced oxidative stress, glomerular hypertrophy, and ECM molecule expression. Pyruvate exhibited a renoprotective effect in the progression of experimental diabetic nephropathy. Future research is warranted to investigate the protective mechanism of pyruvate more specifically in relation with NADPH oxidase in diabetic nephropathy. |
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Authors:
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Kyung Don Ju; Eun Kyoung Shin; Eun Jin Cho; Hyun Bae Yoon; Hyo Sang Kim; Hwajung Kim; Jaeseok Yang; Young-Hwan Hwang; Curie Ahn; Kook-Hwan Oh |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-11-30 |
Journal Detail:
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Title: American journal of physiology. Renal physiology Volume: - ISSN: 1522-1466 ISO Abbreviation: - Publication Date: 2011 Nov |
Date Detail:
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Created Date: 2011-12-1 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100901990 Medline TA: Am J Physiol Renal Physiol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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1Seoul National University Hospital. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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