| Ethyl acetate extract and its major constituent, isorhamnetin 3-O-rutinoside, from Nitraria retusa leaves, promote apoptosis of human myelogenous erythroleukaemia cells. | |
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MedLine Citation:
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PMID: 21951288 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Objective: Fractionation of ethyl acetate extract (EA) obtained from Nitraria retusa leaves was assessed using different methods of chromatography, and isorhamnetin3-O-rutinoside (I3-O-R) was isolated from this extract. Its structure was determined using data obtained from (1) H and (13) C NMR spectra, as well as by various correlation experiments (COSY, HMQC and HMBC). Both EA extract and I3-O-R were investigated for their ability to induce apoptosis in human chronic myelogenous erythroleukaemia cells (K562). Materials and methods: Apoptosis of cells from the K562 line was detected by DNA fragmentation, PARP cleavage and by evaluating activities of caspases 3 and 8. Results: Apoptosis, revealed by DNA fragmentation and PARP cleavage, was observed after 48-h incubation of these human myelogenous erythroleukaemia cells (K562), with the tested products. Likewise, caspase 3 and caspase 8 activities were induced in the presence of the EA extract and I3-O-R after 48 h of incubation. Conclusion: Our results strongly suggest the involvement of the extrinsic pathway of apoptosis in cells treated by both the original EA extract and its major component, I3-O-R. |
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Authors:
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J Boubaker; W Bhouri; M Ben Sghaier; K Ghedira; M G Dijoux Franca; L Chekir-Ghedira |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Cell proliferation Volume: 44 ISSN: 1365-2184 ISO Abbreviation: Cell Prolif. Publication Date: 2011 Oct |
Date Detail:
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Created Date: 2011-09-28 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9105195 Medline TA: Cell Prolif Country: England |
Other Details:
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Languages: eng Pagination: 453-61 Citation Subset: IM |
Copyright Information:
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© 2011 Blackwell Publishing Ltd. |
Affiliation:
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Laboratory of Cellular and Molecular Biology, Faculty of Dental Medicine, University of Monastir, Monastir, Tunisia Unity of Pharmacognosy/ Molecular Biology, Faculty of Pharmacy, University of Monastir, Monastir, Tunisia Laboratory of Botanic, Pharmacognosy and Phytotherapy, Microbial Ecology, Institute of Pharmaceutical and Biological Sciences, University Claude Bernard, Lyon Cedex, France. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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