Document Detail

Ethanolic Extract of Fermented Houttuynia cordata Thunb Induces Human Leukemic HL-60 and Molt-4 Cell Apoptosis Via Oxidative Stress and a Mitochondrial Pathway.
MedLine Citation:
PMID:  22393956     Owner:  NLM     Status:  In-Data-Review    
Houttuynia cordata Thunb (HCT) is a medicinal plant of the Saururaceae family which features antimutagenic and antiviral properties. For extraction, the whole plants were fermented or non-fermented with yeast and ethanol then the whole plants were dried, ground and extracted with 95% ethanol or water. The aims of this study were to compare cytotoxic effects, apoptosis induction, and mechanism(s) with the ethanolic and water extracts of fermented and non-fermented HCT. Cytotoxicity was assessed using the MTT assay in human leukemic HL-60, Molt-4 and peripheral blood mononuclear cells (PBMCs). Apoptotic death was characterized by staining with propidium iodide and examined under a fluorescence microscope. Peroxide radical production and reduction of mitochondrial transmembrane potential (MTP) were determined using 2',7'-dichlorohydrofluorescein diacetate and 3,3'-dihexyloxacarbocyanine iodide and flow cytometry, respectively. The expression of caspase-9 was identified by immunoblotting. The ethanolic extract of fermented HCT was cytotoxic to HL-60 >Molt- 4 > PBMCs, to a greater extent than the non-fermented preparation and the number of apoptotic cells was higher. The alcoholic (fermented) extract produced more radicals than the non-fermented in HL-60 cells but the converse was observed in Molt-4 cells. Reduction of MTP was found in HL-60 and Molt-4 cells treated with the alcoholic (fermented) extract and caspase-9 was cleaved dose-dependently in both cells. In conclusion, the alcoholic extract of fermented HCT was more toxic to human leukemic cells than the non-fermented and both cell lines underwent apoptosis via oxidative stress and a mitochondrial pathway.
Ratana Banjerdpongchai; Prachya Kongtawelert
Related Documents :
12682676 - Immunohistochemical and morphometric study of the development of fetal and newborn rat ...
2907396 - Immunocytochemical localization of peptides in the endocrine pancreas of the snakes vip...
8348756 - Protection of islet cells from inflammatory cell death in vitro.
18381746 - Periostin, secreted from stromal cells, has biphasic effect on cell migration and corre...
7473416 - Ratio of inner cell mass and trophoblastic cells in demi- and intact pig embryos.
3679416 - Cellular changes induced by the tobacco-specific carcinogen 4-(n-nitrosomethylamino)-1-...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Asian Pacific journal of cancer prevention : APJCP     Volume:  12     ISSN:  1513-7368     ISO Abbreviation:  Asian Pac. J. Cancer Prev.     Publication Date:  2011  
Date Detail:
Created Date:  2012-03-07     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101130625     Medline TA:  Asian Pac J Cancer Prev     Country:  Thailand    
Other Details:
Languages:  eng     Pagination:  2871-4     Citation Subset:  IM    
Thailand Excellence Center for Tissue Engineering and Stem Cells, Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand E-mail
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  On-schedule Mammography Rescreening in the National Cancer Screening Program for Breast Cancer in Ko...
Next Document:  Binding Capacity of ER-? Ligands and SERMs: Comparison of the Human, Dog and Cat.